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Chrysanthemum morifoliumFlower Extract Inhibits Adipogenesis of 3T3-L1 Cells via AMPK/SIRT1 Pathway Activation

Title
Chrysanthemum morifoliumFlower Extract Inhibits Adipogenesis of 3T3-L1 Cells via AMPK/SIRT1 Pathway Activation
Authors
Lee, Mak-SoonKim, Yangha
Ewha Authors
김양하
SCOPUS Author ID
김양하scopusscopus
Issue Date
2020
Journal Title
NUTRIENTS
ISSN
2072-6643JCR Link
Citation
NUTRIENTS vol. 12, no. 9
Keywords
Chrysanthemum morifoliumfloweradipogenesisAMPKSIRT1
Publisher
MDPI
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Chrysanthemum (Chrysanthemum morifoliumRamat) flowers (CF) are widely consumed as herbal tea in many countries, including China. The aim of the present study was to examine the anti-adipogenic effect of hot water extraction of CF (HCF) on 3T3-L1 cells and their underlying cellular mechanisms. HCF treatment inhibited lipid accumulation under conditions that did not show the toxicity of 3T3-L1 adipocytes. The activity of glycerol-3-phosphate dehydrogenase (GPDH), which plays an important role in glycerol lipid metabolism, was also reduced by HCF. Adipogenesis/lipogenesis-related mRNA expression levels of peroxisome proliferator-activated receptor-gamma (PPAR-gamma), CCAAT/enhancer-binding protein-alpha (CEBP-alpha), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid-binding protein 4 (FABP4), acetyl-CoA carboxylase 1 (ACC1), and fatty acid synthase (FAS) were suppressed by HCF in a dose-dependent manner. Moreover, HCF increased activities of AMP-activated protein kinase (AMPK) and sirtuin 1 (SIRT1), involved in lipid metabolism. These findings suggest that HCF inhibits adipocyte lipid accumulation through suppression of adipogenesis/lipogenesis-related gene expression and activation of the AMPK/SIRT1 pathway. Therefore, it suggests that HCF may be used as a potentially beneficial plant material for preventing obesity.
DOI
10.3390/nu12092726
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신산업융합대학 > 식품영양학과 > Journal papers
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