View : 742 Download: 0
The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis
- Title
- The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis
- Authors
- Moon J.Y.; Mosaddik A.; Kim H.; Cho M.; Choi H.-K.; Kim Y.S.; Cho S.K.
- Ewha Authors
- 김영석
- SCOPUS Author ID
- 김영석


- Issue Date
- 2011
- Journal Title
- Food Chemistry
- ISSN
- 0308-8146
- Citation
- Food Chemistry vol. 125, no. 2, pp. 369 - 375
- Indexed
- SCI; SCIE; SCOPUS

- Document Type
- Article
- Abstract
- The antiproliferative activities of the chloroform fraction (CF) of guava (Psidium cattleianum Sabine) leaf extract were evaluated using several cancer cell lines. Maximum cytotoxicity was observed in SNU-16, a human gastric carcinoma cell line, at concentrations of 50-100 μg/ml. Flow cytometric analysis demonstrated that CF treatment resulted in a marked accumulation of SNU-16 cells in the sub-G1 phase at concentrations of 100-200 μg/ml. The induction of apoptosis in SNU-16 cells was confirmed by immunoblotting using antibodies against Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-8, and caspase-3. The major CF phytochemicals were identified as ferulic acid, genistein, 3′, 4′, 5′ trimethoxy flavone, phlorizin, and oleanolic acid by high performance liquid chromatography coupled with a photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS). The results suggest that phytochemicals in the CF of guava (P. cattleianum) leaf extract induce apoptosis in SNU-16 cells. These findings may lead to new strategies for treating human gastric cancer. © 2010 Elsevier Ltd.
- DOI
- 10.1016/j.foodchem.2010.09.007
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML