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Cytoprotection of Probiotic Lactobacillus acidophilus with Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation
- Title
- Cytoprotection of Probiotic Lactobacillus acidophilus with Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation
- Authors
- Han, Sang Yeong; Nguyen, Duc Tai; Kim, Beom Jin; Kim, Nayoung; Kang, Eunhye K.; Park, Ji Hun; Choi, Insung S.
- Ewha Authors
- 박지훈
- SCOPUS Author ID
- 박지훈
- Issue Date
- 2023
- Journal Title
- POLYMERS
- ISSN
- 2073-4360
- Citation
- POLYMERS vol. 15, no. 5
- Keywords
- coffee melanoidins; eggshell membrane hydrolysates; Lactobacillus acidophilus; single-cell nanoencapsulation; probiotics
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- One-step fabrication method for thin films and shells is developed with nature-derived eggshell membrane hydrolysates (ESMHs) and coffee melanoidins (CMs) that have been discarded as food waste. The nature-derived polymeric materials, ESMHs and CMs, prove highly biocompatible with living cells, and the one-step method enables cytocompatible construction of cell-in-shell nanobiohybrid structures. Nanometric ESMH-CM shells are formed on individual probiotic Lactobacillus acidophilus, without any noticeable decrease in viability, and the ESMH-CM shells effectively protected L. acidophilus in the simulated gastric fluid (SGF). The cytoprotection power is further enhanced by Fe3+-mediated shell augmentation. For example, after 2 h of incubation in SGF, the viability of native L. acidophilus is 30%, whereas nanoencapsulated L. acidophilus, armed with the Fe3+-fortified ESMH-CM shells, show 79% in viability. The simple, time-efficient, and easy-to-process method developed in this work would contribute to many technological developments, including microbial biotherapeutics, as well as waste upcycling.
- DOI
- 10.3390/polym15051104|http://dx.doi.org/10.3390/polym15051104
- Appears in Collections:
- 사범대학 > 과학교육과 > Journal papers
- Files in This Item:
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polymers-15-01104-v3.pdf(1.67 MB)
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