View : 3 Download: 0

Optimization of leucrose production by dextransucrase from Streptococcus mutans and its application as an adipogenesis regulator

Title
Optimization of leucrose production by dextransucrase from Streptococcus mutans and its application as an adipogenesis regulator
Authors
Lee, DaeyeonLee, JihyeHong, Moon-GiLee, Byung-HooKim, Young-MinChang, Pahn-ShickKim, YuriYoo, Sang-Ho
Ewha Authors
김유리
SCOPUS Author ID
김유리scopus
Issue Date
2017
Journal Title
JOURNAL OF FUNCTIONAL FOODS
ISSN
1756-4646JCR Link
Citation
vol. 39, pp. 238 - 244
Keywords
LeucroseSucroseDextransucraseStreptococcus mutansAdipogenesis
Publisher
ELSEVIER SCIENCE BV
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Leucrose is a sucrose isomer which has an alpha-1,5-linkage, and slowly hydrolyzed to glucose and fructose by small intestinal alpha-glucosidases. Leucrose can be produced by an isomerization reaction of dextransucrase on a sucrose substrate. In this study, the recombinant dextransucrase from Streptococcus mutans (SmDS) was applied to optimize the reaction conditions for leucrose production. With a substrate mixture of 0.5 M sucrose + 1.0 M fructose, the greatest yield (ca. 24.5%) of leucrose was obtained by SIDS treatment at 30 degrees C for 120 h. When preadipocyte 3T3-L1 cells were treated with leucrose, this disaccharide inhibited intracellular lipid accumulation in a dose-dependent manner and significantly suppressed mRNA levels of major adipogenic genes, including CCAT/enhancer-binding protein alpha (C/EBP alpha), peroxisome proliferator-activated receptor-gamma (PPAR gamma), fatty acid synthase (FAS), and sterol regulatory element-binding protein-1C (SREBP-1C). Phosphorylation of P13 kinase/Akt/mTOR was also reduced with leucrose treatment. These results suggest that leucrose has a potential in regulating adipogenesis.
DOI
10.1016/j.jff.2017.10.019
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
Files in This Item:
There are no files associated with this item.


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE