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Optimization of leucrose production by dextransucrase from Streptococcus mutans and its application as an adipogenesis regulator
- Title
- Optimization of leucrose production by dextransucrase from Streptococcus mutans and its application as an adipogenesis regulator
- Authors
- Lee, Daeyeon; Lee, Jihye; Hong, Moon-Gi; Lee, Byung-Hoo; Kim, Young-Min; Chang, Pahn-Shick; Kim, Yuri; Yoo, Sang-Ho
- Ewha Authors
- 김유리
- SCOPUS Author ID
- 김유리
- Issue Date
- 2017
- Journal Title
- JOURNAL OF FUNCTIONAL FOODS
- ISSN
- 1756-4646
- Citation
- JOURNAL OF FUNCTIONAL FOODS vol. 39, pp. 238 - 244
- Keywords
- Leucrose; Sucrose; Dextransucrase; Streptococcus mutans; Adipogenesis
- Publisher
- ELSEVIER SCIENCE BV
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Leucrose is a sucrose isomer which has an alpha-1,5-linkage, and slowly hydrolyzed to glucose and fructose by small intestinal alpha-glucosidases. Leucrose can be produced by an isomerization reaction of dextransucrase on a sucrose substrate. In this study, the recombinant dextransucrase from Streptococcus mutans (SmDS) was applied to optimize the reaction conditions for leucrose production. With a substrate mixture of 0.5 M sucrose + 1.0 M fructose, the greatest yield (ca. 24.5%) of leucrose was obtained by SIDS treatment at 30 degrees C for 120 h. When preadipocyte 3T3-L1 cells were treated with leucrose, this disaccharide inhibited intracellular lipid accumulation in a dose-dependent manner and significantly suppressed mRNA levels of major adipogenic genes, including CCAT/enhancer-binding protein alpha (C/EBP alpha), peroxisome proliferator-activated receptor-gamma (PPAR gamma), fatty acid synthase (FAS), and sterol regulatory element-binding protein-1C (SREBP-1C). Phosphorylation of P13 kinase/Akt/mTOR was also reduced with leucrose treatment. These results suggest that leucrose has a potential in regulating adipogenesis.
- DOI
- 10.1016/j.jff.2017.10.019
- Appears in Collections:
- 신산업융합대학 > 식품영양학과 > Journal papers
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