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Green tea (-)-epigallotocatechin-3-gallate induces pgc-1α gene expression in HepG2 cells and 3T3-L1 adipocytes

Title
Green tea (-)-epigallotocatechin-3-gallate induces pgc-1α gene expression in HepG2 cells and 3T3-L1 adipocytes
Authors
Lee M.-S.Lee S.Doo M.Kim Y.
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2016
Journal Title
Preventive Nutrition and Food Science
ISSN
2287-1098JCR Link
Citation
Preventive Nutrition and Food Science vol. 21, no. 1, pp. 62 - 67
Keywords
3T3-L1 adipocytesEGCGHepG2 cellsPGC-1αPromoter activity
Publisher
Korean Society of Food Science and Nutrition
Indexed
SCOPUS; KCI scopus
Document Type
Article
Abstract
Green tea (Camellia sinensis) is one of the most popular beverages in the world and has been acknowledged for centuries as having significant health benefits. (ï)-Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea, and it has been reported to have health benefit effects. Peroxisome proliferator-activated receptor γ coactivator (PGC)-1α is a crucial regulator of mitochondrial biogenesis and hepatic gluconeogenesis. The objective of this study was to investigate whether EGCG from green tea can affect the ability of transcriptional regulation on PGC-1α mRNA expression in HepG2 cells and 3T3-L1 adipocytes. To study the molecular mechanism that allows EGCG to control PGC-1α expression, the promoter activity levels of PGC-1α were examined. The PGC-1α mRNA level was measured using quantitative real-time PCR. The -970/+412 bp of PGC-1α promoter was subcloned into the pGL3-Basic vector that includes luciferase as a reporter gene. EGCG was found to up-regulate the PGC-1α mRNA levels significantly with 10 μmol/L of EGCG in HepG2 cells and differentiated 3T3-L1 adipocytes. PGC-1α promoter activity was also increased by treatment with 10 μmol/L of EGCG in both cells. These results suggest that EGCG may induce PGC-1α gene expression, potentially through promoter activation. © Copyright 2016 by The Korean Society of Food Science and Nutrition. All rights Reserved.
DOI
10.3746/pnf.2016.21.1.62
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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