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Green tea (-)-epigallotocatechin-3-gallate induces pgc-1α gene expression in HepG2 cells and 3T3-L1 adipocytes

Title
Green tea (-)-epigallotocatechin-3-gallate induces pgc-1α gene expression in HepG2 cells and 3T3-L1 adipocytes
Authors
Lee M.-S.Lee S.Doo M.Kim Y.
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2016
Journal Title
Preventive Nutrition and Food Science
ISSN
2287-1098JCR Link
Citation
vol. 21, no. 1, pp. 62 - 67
Keywords
3T3-L1 adipocytesEGCGHepG2 cellsPGC-1αPromoter activity
Publisher
Korean Society of Food Science and Nutrition
Indexed
SCOPUS; KCI scopus
Abstract
Green tea (Camellia sinensis) is one of the most popular beverages in the world and has been acknowledged for centuries as having significant health benefits. (ï)-Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea, and it has been reported to have health benefit effects. Peroxisome proliferator-activated receptor γ coactivator (PGC)-1α is a crucial regulator of mitochondrial biogenesis and hepatic gluconeogenesis. The objective of this study was to investigate whether EGCG from green tea can affect the ability of transcriptional regulation on PGC-1α mRNA expression in HepG2 cells and 3T3-L1 adipocytes. To study the molecular mechanism that allows EGCG to control PGC-1α expression, the promoter activity levels of PGC-1α were examined. The PGC-1α mRNA level was measured using quantitative real-time PCR. The -970/+412 bp of PGC-1α promoter was subcloned into the pGL3-Basic vector that includes luciferase as a reporter gene. EGCG was found to up-regulate the PGC-1α mRNA levels significantly with 10 μmol/L of EGCG in HepG2 cells and differentiated 3T3-L1 adipocytes. PGC-1α promoter activity was also increased by treatment with 10 μmol/L of EGCG in both cells. These results suggest that EGCG may induce PGC-1α gene expression, potentially through promoter activation. © Copyright 2016 by The Korean Society of Food Science and Nutrition. All rights Reserved.
DOI
10.3746/pnf.2016.21.1.62
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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