Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김양하 | * |
dc.date.accessioned | 2016-08-29T12:08:45Z | - |
dc.date.available | 2016-08-29T12:08:45Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 2287-1098 | * |
dc.identifier.other | OAK-18525 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231521 | - |
dc.description.abstract | Green tea (Camellia sinensis) is one of the most popular beverages in the world and has been acknowledged for centuries as having significant health benefits. (ï)-Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea, and it has been reported to have health benefit effects. Peroxisome proliferator-activated receptor γ coactivator (PGC)-1α is a crucial regulator of mitochondrial biogenesis and hepatic gluconeogenesis. The objective of this study was to investigate whether EGCG from green tea can affect the ability of transcriptional regulation on PGC-1α mRNA expression in HepG2 cells and 3T3-L1 adipocytes. To study the molecular mechanism that allows EGCG to control PGC-1α expression, the promoter activity levels of PGC-1α were examined. The PGC-1α mRNA level was measured using quantitative real-time PCR. The -970/+412 bp of PGC-1α promoter was subcloned into the pGL3-Basic vector that includes luciferase as a reporter gene. EGCG was found to up-regulate the PGC-1α mRNA levels significantly with 10 μmol/L of EGCG in HepG2 cells and differentiated 3T3-L1 adipocytes. PGC-1α promoter activity was also increased by treatment with 10 μmol/L of EGCG in both cells. These results suggest that EGCG may induce PGC-1α gene expression, potentially through promoter activation. © Copyright 2016 by The Korean Society of Food Science and Nutrition. All rights Reserved. | * |
dc.language | English | * |
dc.publisher | Korean Society of Food Science and Nutrition | * |
dc.subject | 3T3-L1 adipocytes | * |
dc.subject | EGCG | * |
dc.subject | HepG2 cells | * |
dc.subject | PGC-1α | * |
dc.subject | Promoter activity | * |
dc.title | Green tea (-)-epigallotocatechin-3-gallate induces pgc-1α gene expression in HepG2 cells and 3T3-L1 adipocytes | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 21 | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 62 | * |
dc.relation.lastpage | 67 | * |
dc.relation.journaltitle | Preventive Nutrition and Food Science | * |
dc.identifier.doi | 10.3746/pnf.2016.21.1.62 | * |
dc.identifier.scopusid | 2-s2.0-84963612618 | * |
dc.author.google | Lee M.-S. | * |
dc.author.google | Lee S. | * |
dc.author.google | Doo M. | * |
dc.author.google | Kim Y. | * |
dc.contributor.scopusid | 김양하(8524160300) | * |
dc.date.modifydate | 20240123090847 | * |