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Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification

Title
Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification
Authors
Lee, Eun-JuLee, Moon WonNo, Da SomKim, Hee-JinOh, Se-WookKim, YanghaKim, In-Hwan
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2016
Journal Title
JOURNAL OF FUNCTIONAL FOODS
ISSN
1756-4646JCR Link
Citation
vol. 21, pp. 330 - 337
Keywords
Crypthecodinium cohniiDocosahexaenoic acid (DHA)EsterificationLipaseMicroalgaeRhizomucor miehei
Publisher
ELSEVIER SCIENCE BV
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 degrees C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min. (C) 2015 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.jff.2015.12.020
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신산업융합대학 > 식품영양학과 > Journal papers
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