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beta-glucan suppresses LPS-stimulated NO production through the down-regulation of iNOS expression and NF kappa B Transactivation in RAW 264.7 macrophages

Title
beta-glucan suppresses LPS-stimulated NO production through the down-regulation of iNOS expression and NF kappa B Transactivation in RAW 264.7 macrophages
Authors
Yang, Jeong-LyeJang, Ji-HyunRadhakrishnan, VinodhkurnarVjm, Yang-HaSong, Young-Sun
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2008
Journal Title
FOOD SCIENCE AND BIOTECHNOLOGY
ISSN
1226-7708JCR Link
Citation
vol. 17, no. 1, pp. 106 - 113
Keywords
barley beta-glucanoxidative stressnuclear factor kappa Binducible nitric oxide synthase expressionnitric oxidemacrophage
Publisher
KOREAN SOC FOOD SCIENCE TECHNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The antioxidant and anti-inflammatory protective effects of beta-glucan from barley on RAW 264.7 murine macrophage cells induced by lipopolysaccharide (LPS) were examined. The RAW 264.7 murine macrophages were preincubated with various concentrations (0-200 mu g/mL) of beta-glucan and stimulated with LPS to induce oxidative stress and inflammation. The beta-glucan treatments were found to reduce thiobarbituric acid-reactive substance (TBARS) accumulation, and enhance glutathione levels and the activities of antioxidative enzymes, including superoxide dismutase (SOD), catalase, glutathione reductase, and glutathione peroxidase (GSH-px) in the LPS-stimulated macrophages as compared to the LPS-only treated cells. Nitric oxide (NO) production was significantly suppressed in a dose-dependent manner (p<0.05) with an IC50 of 104 mu g/mL. Further treatment with beta-glucan at 200 mu g/mL suppressed NO production to 2% of the LPS-control, and suppressed the levels of inducible nitric oxide synthase (NOS) protein and mRNA in a dose-dependent manner. The specific DNA binding activity of nuclear factor kappa B (NF kappa B) was significantly suppressed by beta-glucan treatment with an IC50 of 220 mu g/mL in a dose-dependent manner. Finally, barley beta-glucan ameliorates NO production and iNOS expression through the down-regulation of NF kappa B activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages.
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신산업융합대학 > 식품영양학과 > Journal papers
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