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dc.contributor.author김양하-
dc.date.accessioned2016-08-27T02:08:49Z-
dc.date.available2016-08-27T02:08:49Z-
dc.date.issued2008-
dc.identifier.issn1226-7708-
dc.identifier.otherOAK-4669-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/216225-
dc.description.abstractThe antioxidant and anti-inflammatory protective effects of beta-glucan from barley on RAW 264.7 murine macrophage cells induced by lipopolysaccharide (LPS) were examined. The RAW 264.7 murine macrophages were preincubated with various concentrations (0-200 mu g/mL) of beta-glucan and stimulated with LPS to induce oxidative stress and inflammation. The beta-glucan treatments were found to reduce thiobarbituric acid-reactive substance (TBARS) accumulation, and enhance glutathione levels and the activities of antioxidative enzymes, including superoxide dismutase (SOD), catalase, glutathione reductase, and glutathione peroxidase (GSH-px) in the LPS-stimulated macrophages as compared to the LPS-only treated cells. Nitric oxide (NO) production was significantly suppressed in a dose-dependent manner (p<0.05) with an IC50 of 104 mu g/mL. Further treatment with beta-glucan at 200 mu g/mL suppressed NO production to 2% of the LPS-control, and suppressed the levels of inducible nitric oxide synthase (NOS) protein and mRNA in a dose-dependent manner. The specific DNA binding activity of nuclear factor kappa B (NF kappa B) was significantly suppressed by beta-glucan treatment with an IC50 of 220 mu g/mL in a dose-dependent manner. Finally, barley beta-glucan ameliorates NO production and iNOS expression through the down-regulation of NF kappa B activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages.-
dc.languageEnglish-
dc.publisherKOREAN SOC FOOD SCIENCE TECHNOLOGY-
dc.subjectbarley beta-glucan-
dc.subjectoxidative stress-
dc.subjectnuclear factor kappa B-
dc.subjectinducible nitric oxide synthase expression-
dc.subjectnitric oxide-
dc.subjectmacrophage-
dc.titlebeta-glucan suppresses LPS-stimulated NO production through the down-regulation of iNOS expression and NF kappa B Transactivation in RAW 264.7 macrophages-
dc.typeArticle-
dc.relation.issue1-
dc.relation.volume17-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.indexKCI-
dc.relation.startpage106-
dc.relation.lastpage113-
dc.relation.journaltitleFOOD SCIENCE AND BIOTECHNOLOGY-
dc.identifier.wosidWOS:000253922600018-
dc.identifier.scopusid2-s2.0-49649117730-
dc.author.googleYang, Jeong-Lye-
dc.author.googleJang, Ji-Hyun-
dc.author.googleRadhakrishnan, Vinodhkurnar-
dc.author.googleVjm, Yang-Ha-
dc.author.googleSong, Young-Sun-
dc.contributor.scopusid김양하(8524160300)-
dc.date.modifydate20170508110102-
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신산업융합대학 > 식품영양학과 > Journal papers
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