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Multilayer Engineering of an Escherichia coli-Based Biotransformation System to Exclusively Produce Glycolic Acid from Formaldehyde
- Title
- Multilayer Engineering of an Escherichia coli-Based Biotransformation System to Exclusively Produce Glycolic Acid from Formaldehyde
- Authors
- Jo, Hye-Jin; Yu, Hanna; Cheon, Huijin; Kim, Jun-Hong; Kim, Ga Young; Kim, Byoung Sik; Kim, Jeong-Sun; Park, Jin-Byung
- Ewha Authors
- 박진병; 김병식
- SCOPUS Author ID
- 박진병; 김병식
- Journal Title
- ACS SUSTAINABLE CHEMISTRY & ENGINEERING
- ISSN
- 2168-0485
- Citation
- ACS SUSTAINABLE CHEMISTRY & ENGINEERING
- Keywords
- Whole-cell biocatalysis; Carboligase; Aldehyde dehydrogenase; Glycolic acid; Formaldehyde; Escherichia coli
- Publisher
- AMER CHEMICAL SOC
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
Early Access
- Abstract
- A whole-cell biocatalysis was investigated for the selective synthesis of the industrially relevant C2 chemicals (e.g., glycolic acid (3)) from formaldehyde (1). Escherichia coli cells were engineered to overexpress a carboligase and an aldehyde dehydrogenase from E. coli K-12. Moreover, the side reactions, which dissipate formaldehyde and glycolic acid, were removed to produce glycolic acid to a high conversion. Host cell engineering to apply relatively chemical tolerant E. coli strains as well as substrate engineering to avoid the toxic effects of formaldehyde to the host cells allowed production of glycolic acid up to 27 mM in the reaction medium with a conversion of 85%. This study will contribute to valorization of C1 gas (e.g., CH4, CO2, and CO) to industrially relevant C2 chemicals in a sustainable way.
- DOI
- 10.1021/acssuschemeng.2c05936
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
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