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Walnut phenolic extracts reduce telomere length and telomerase activity in a colon cancer stem cell model

Title
Walnut phenolic extracts reduce telomere length and telomerase activity in a colon cancer stem cell model
Authors
Shin, Phil-KyungZoh, YoonchaeChoi, JinaKim, Myung-SunnyKim, YuriChoi, Sang-Woon
Ewha Authors
김유리
SCOPUS Author ID
김유리scopus
Issue Date
2019
Journal Title
NUTRITION RESEARCH AND PRACTICE
ISSN
1976-1457JCR Link

2005-6168JCR Link
Citation
NUTRITION RESEARCH AND PRACTICE vol. 13, no. 1, pp. 58 - 63
Keywords
Walnutcolonic neoplasmsstem cellstelomeretelomerase
Publisher
KOREAN NUTRITION SOC
Indexed
SCIE; SCOPUS; KCI WOS
Document Type
Article
Abstract
BACKGROUND/OBJECTIVES: Telomeres are located at the chromosomal ends and progressively shortened during each cell cycle. Telomerase, which is regulated by hTERT and c-MYC, maintains telomeric DNA sequences. Especially, telomerase is active in cancer and stem cells to maintain telomere length for replicative immortality. Recently we reported that walnut phenolic extract (WPE) can reduce cell viability in a colon cancer stem cell (CSC) model. We, therefore, investigated the effect of WPE on telomere maintenance in the same model. MATERIALS AND METHODS: CD133(+) CD44(+) cells from HCT116, a human colon cancer cell line, were sorted by Fluorescence-activated cell sorting (FACS) and treated with WPE at the concentrations of 0, 10, 20, and 40 mu g/mL for 6 days. Telomere lengths were assessed by quantitative real-time PCR (qRT-PCR) using telomere specific primers and DNA extracted from the cells, which was further adjusted with single-copy gene and reference DNA (ddC(t)). Telomerase activity was also measured by qRT-PCR after incubating the PCR mixture with cell protein extracts, which was adjusted with reference DNA (dC(t)). Transcriptions of hTERT and c-MYC were determined using conventional RT-PCR. RESULTS: Telomere length of WPE-treated cells was significantly decreased in a dose-dependent manner (5.16 +/- 0.13 at 0 mu g/mL, 4.79 +/- 0.12 at 10 pg/mL, 3.24 +/- 0.08 at 20 mu g/mL and 3.99 +/- 0.09 at 40 mu g/mL; P=0.0276). Telomerase activities concurrently decreased with telomere length (1.47 +/- 0.04, 1.09 +/- 0.01, 0.76 +/- 0.08, and 0.88 +/- 0.06; P = 0.0067). There was a positive correlation between telomere length and telomerase activity (r = 0.9090; P < 0.0001). Transcriptions of both hTERT and c-MYC were also significantly decreased in the same manner. CONCLUSION: In the present cell culture model, WPE reduced telomere maintenance, which may provide a mechanistic link to the effect of walnuts on the viability of colon CSCs.
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DOI
10.4162/nrp.2019.13.1.58
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신산업융합대학 > 식품영양학과 > Journal papers
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