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Synergistic cranberry juice combinations with natural-borne antimicrobials for the eradication of uropathogenic Escherichia coli biofilm within a short time

Title
Synergistic cranberry juice combinations with natural-borne antimicrobials for the eradication of uropathogenic Escherichia coli biofilm within a short time
Authors
Kim, H. W.Chung, D. H.Kim, S. A.Rhee, M. S.
Ewha Authors
김선애
SCOPUS Author ID
김선애scopus
Issue Date
2019
Journal Title
LETTERS IN APPLIED MICROBIOLOGY
ISSN
0266-8254JCR Link

1472-765XJCR Link
Citation
LETTERS IN APPLIED MICROBIOLOGY vol. 68, no. 4, pp. 321 - 328
Keywords
biofilmscaprylic acidcranberrythymolurinary tract infectionsuropathogenic Escherichia coli
Publisher
WILEY
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Urinary tract infections (UTI), one of the most common diseases in humans, are caused primarily by uropathogenic Escherichia coli (UPEC). Cranberry juice (CB) is a widely known prophylaxis for UTI, but the treatment of CB alone could not effectively eradicate preformed UPEC biofilms. The aim of this study was to develop enforced CB composites within a short time by adding a small quantity of natural borne antimicrobials. UPEC biofilms (initial: 6 center dot 0 log CFU per cm(2)), formed on silicone coupons in artificial urine medium, were exposed to CB (4-8%), caprylic acid (CAR; 0 center dot 025-0 center dot 05%) and thymol (TM; 0 center dot 025-0 center dot 05%) at 37 degrees C for 1 min. Individual treatment of each compound did not show the significant antibacterial effect on UPEC biofilms (P > 0 center dot 05). Otherwise, the survivor counts of biofilms were synergistically reduced with CB containing any of the antimicrobials. For example combined treatment with CB (8%) + CAR (0 center dot 05%) + TM (0 center dot 05%) resulted in a 6 log reduction in UPEC populations in the biofilm (no detectable bacteria remained) with 4 center dot 6 log of synergistic bactericidal effect. The confocal laser scanning microscope images indicated that any composites including TM might result in biofilm detachment from the surface. The present method is cost-effective and more acceptable to consumers as it is based on the synergistic interaction of natural borne antimicrobials. The results of this study could be widely applicable in the functional food, medical and healthcare field. Significance and Impact of the Study Anti-biofilm effect of cranberry juice (CB) has been focused mainly on inhibiting biofilm formation of uropathogenic Escherichia coli (UPEC); however, combined treatment with natural borne antimicrobials derived from coconut oil (caprylic acid) and oregano essential oil (thymol) could synergistically enhance its eradicating activity against biofilms. This study developed novel CB composites showing marked anti-biofilm effects (complete eradication of UPEC biofilms within just 1 min).
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DOI
10.1111/lam.13140
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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