Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 홍재희 | * |
dc.date.accessioned | 2016-08-29T11:08:20Z | - |
dc.date.available | 2016-08-29T11:08:20Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 0021-8561 | * |
dc.identifier.other | OAK-5792 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232016 | - |
dc.description.abstract | Interaction of taste molecules with saliva is the first step in the flavor perception process. Saliva is assumed to influence copper-induced sensation by controlling the copper solubility or causing astringency via binding of proteins with copper. This study was performed to identify the nature of copper-protein interactions in relation to the sensory perception of copper. Saliva was treated with CuSO 4 · 5H 2O at levels of 0, 2.5, 5, 10, 20, or 40 mg/L, and changes in salivary proteins were analyzed using high performance liquid chromatography (HPLC) and sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). Protein peaks that showed changes in HPLC were characterized with SDS-PAGE. HPLC analysis revealed that copper treatment up to 40 mg/L decreased several proteins, including the dominant peak, by 70%. This peak was composed of α-amylase, a secretory component, and basic proline-rich proteins. SDS-PAGE results showed that salivary proteins of molecular weight 29 kDa and 33 kDa precipitated when copper was added at concentrations ≥10 mg/L. This study provides biochemical information for understanding perception mechanisms of copper sensation. © 2009 American Chemical Society. | * |
dc.language | English | * |
dc.title | Interaction of copper and human salivary proteins | * |
dc.type | Article | * |
dc.relation.issue | 15 | * |
dc.relation.volume | 57 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 6967 | * |
dc.relation.lastpage | 6975 | * |
dc.relation.journaltitle | Journal of Agricultural and Food Chemistry | * |
dc.identifier.doi | 10.1021/jf804047h | * |
dc.identifier.wosid | WOS:000268537300067 | * |
dc.identifier.scopusid | 2-s2.0-68249110178 | * |
dc.author.google | Hee Hong J.A.E. | * |
dc.author.google | Duncan S.E. | * |
dc.author.google | Dietrich A.M. | * |
dc.author.google | O'Keefe S.F. | * |
dc.author.google | Eigel W.N. | * |
dc.author.google | Mallikarjunan K. | * |
dc.contributor.scopusid | 홍재희(24764545900) | * |
dc.date.modifydate | 20240322142021 | * |