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Prevalence and characterization of typical aeromonas salmonicida chum salmon isolates in Korea
- Prevalence and characterization of typical aeromonas salmonicida chum salmon isolates in Korea
- Kim Y.-S.; Yoon J.W.; Han H.-J.; Suebsing R.; Kim J.-H.
- Ewha Authors
- Issue Date
- Journal Title
- Fisheries and Aquatic Science
- Fisheries and Aquatic Science vol. 14, no. 4, pp. 347 - 354
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- Aeromonas salmonicida is an important fish pathogen commonly associated with furunculosis in salmonids. Typical A. salmonicida strains have the surface virulence A-layer protein, a major virulence determinant encoded by the vapA gene. In this study, 880 chum salmon Oncorhynchus keta were collected from the east coast of Korea during 2006-2011, including 560 wild adults and 320 artificially hatched fry pools, and the presence of typical A. salmonicida was examined by PCR using the typical A. salmonicida-specific vapA gene primers. The results demonstrated that 34.5% of the samples (304/880 samples) were PCR positive, implying that a typical A. salmonicida infection is highly prevalent among chum salmon in Korea. Twenty typical A. salmonicida isolates were recovered based on their brown pigmentation on Trypticase Soy Agar (TSA) plates, which indicates the existence of the A-layer protein. Further biochemical analyses with the four randomly selected typical A. salmonicida isolates revealed some variations in their amino acid decarboxylation and carbohydrate fermentation activity. A phylogenetic analysis based on the entire vapA gene sequence suggested that the A. salmonicida isolates from chum salmon were clustered with those isolated from Atlantic salmon in Europe. Further study is needed to resolve such an interesting relationship in detail. © The Korean Society of Fisheries and Aquatic Science.
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