Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박진병 | * |
dc.contributor.author | 윤장원 | * |
dc.contributor.author | 양경미 | * |
dc.date.accessioned | 2016-08-27T02:08:21Z | - |
dc.date.available | 2016-08-27T02:08:21Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 1738-2203 | * |
dc.identifier.other | OAK-8780 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216596 | - |
dc.description.abstract | To isolate a new P450 monooxygenase belonging to the CYP102 family, CYP102H1 of Nocardia farcinica IFM 10152 (i.e., pnf11 580) was cloned, expressed, and partially characterized. CYP102H1 gene was amplified from pNF1 of N farcinica and cloned into expression vectors (i.e., pTrc99A, pET28a(+)). When Escherichia coli BL21(DE3) codon+ strain was transformed with pET28a-CYP102H1 and the culture was induced with 1.0 mM isopropyl-beta-D-thio-galactoside in a complex medium at 30 degrees C, CYP102H1 could be expressed in soluble form even though soluble form was not dominant. The enzyme showed typical features of heme proteins; a spectrum of reduced CO bound form showed typical maximum at 450 nm. When the biotransformation of linoleic acid was carried out in a reconstituted system consisting of CYP102H1 and redox partner proteins of Pseudomonas putida (i.e., CamAB), omega 1-hydroxylinoleic acid was detected with gas chromatography/mass spectrometry analysis, suggesting that CYP102H1 catalyzes oxygenation of linoleic acid at omega-1 position, which is typical in CYP102A subfamily members. | * |
dc.language | English | * |
dc.publisher | KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY | * |
dc.subject | cloning | * |
dc.subject | CYP102H1 | * |
dc.subject | Escherichia coli | * |
dc.subject | Nocardia farcinica | * |
dc.subject | oxygenation | * |
dc.title | Cloning, Expression, and Characterization of P450 Monooxygenase CYP102H1 from Nocardia farcinica | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 55 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 259 | * |
dc.relation.lastpage | 264 | * |
dc.relation.journaltitle | JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY | * |
dc.identifier.doi | 10.1007/s13765-012-1018-8 | * |
dc.identifier.wosid | WOS:000304023400015 | * |
dc.author.google | Chung, Yoon-Hee | * |
dc.author.google | Song, Ji-Won | * |
dc.author.google | Choi, Kwon-Young | * |
dc.author.google | Yoon, Jang Won | * |
dc.author.google | Yang, Kyung-Mi | * |
dc.author.google | Park, Jin-Byung | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.contributor.scopusid | 윤장원(7403587255) | * |
dc.date.modifydate | 20240322114808 | * |