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Microbial Community Analysis of a Methane-Oxidizing Biofilm Using Ribosomal Tag Pyrosequencing

Title
Microbial Community Analysis of a Methane-Oxidizing Biofilm Using Ribosomal Tag Pyrosequencing
Authors
Kim, Tae GwanLee, Eun-HeeCho, Kyung-Suk
Ewha Authors
조경숙김태관이은희
SCOPUS Author ID
조경숙scopus; 이은희scopusscopus
Issue Date
2012
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN
1017-7825JCR Link
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY vol. 22, no. 3, pp. 360 - 370
Keywords
Pyrosequencingcommunity analysismethanotrophsbiofilmquantitative real-time PCR
Publisher
KOREAN SOC MICROBIOLOGY &

BIOTECHNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
Current ecological knowledge of methanotrophic biofilms is incomplete, although they have been broadly studied in biotechnological processes. Four individual DNA samples were prepared from a methanotrophic biofilm, and a multiplex 16S rDNA pyrosequencing was performed. A complete library (before being de-multiplexed) contained 33,639 sequences (average length, 415 nt). Interestingly, methanotrophs were not dominant, only making up 23% of the community. Methylosinus, Methylomonas, and Methylosarcina were the dominant methanotrophs. Type II methanotrophs were more abundant than type (56 vs. 44%), but less richer and diverse. Dominant non-methanotrophic genera included Hydrogenophaga, Flavobacterium, and Hyphomicrobium. The library was de-multiplexed into four libraries, with different sequencing efforts (3,915 - 20,133 sequences). Sorrenson abundance similarity results showed that the four libraries were almost identical (indices > 0.97), and phylogenetic comparisons using UniFrac test and P-test revealed the same results. It was demonstrated that the pyrosequencing was highly reproducible. These survey results can provide an insight into the management and/or manipulation of methanotrophic biofilms.
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DOI
10.4014/jmb.1109.09052
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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