Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조경숙 | * |
dc.contributor.author | 김태관 | * |
dc.contributor.author | 이은희 | * |
dc.date.accessioned | 2016-08-27T02:08:21Z | - |
dc.date.available | 2016-08-27T02:08:21Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 1017-7825 | * |
dc.identifier.other | OAK-8620 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216591 | - |
dc.description.abstract | Current ecological knowledge of methanotrophic biofilms is incomplete, although they have been broadly studied in biotechnological processes. Four individual DNA samples were prepared from a methanotrophic biofilm, and a multiplex 16S rDNA pyrosequencing was performed. A complete library (before being de-multiplexed) contained 33,639 sequences (average length, 415 nt). Interestingly, methanotrophs were not dominant, only making up 23% of the community. Methylosinus, Methylomonas, and Methylosarcina were the dominant methanotrophs. Type II methanotrophs were more abundant than type (56 vs. 44%), but less richer and diverse. Dominant non-methanotrophic genera included Hydrogenophaga, Flavobacterium, and Hyphomicrobium. The library was de-multiplexed into four libraries, with different sequencing efforts (3,915 - 20,133 sequences). Sorrenson abundance similarity results showed that the four libraries were almost identical (indices > 0.97), and phylogenetic comparisons using UniFrac test and P-test revealed the same results. It was demonstrated that the pyrosequencing was highly reproducible. These survey results can provide an insight into the management and/or manipulation of methanotrophic biofilms. | * |
dc.language | English | * |
dc.publisher | KOREAN SOC MICROBIOLOGY & | * |
dc.publisher | BIOTECHNOLOGY | * |
dc.subject | Pyrosequencing | * |
dc.subject | community analysis | * |
dc.subject | methanotrophs | * |
dc.subject | biofilm | * |
dc.subject | quantitative real-time PCR | * |
dc.title | Microbial Community Analysis of a Methane-Oxidizing Biofilm Using Ribosomal Tag Pyrosequencing | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 22 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 360 | * |
dc.relation.lastpage | 370 | * |
dc.relation.journaltitle | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | * |
dc.identifier.doi | 10.4014/jmb.1109.09052 | * |
dc.identifier.wosid | WOS:000302190800011 | * |
dc.identifier.scopusid | 2-s2.0-84859067410 | * |
dc.author.google | Kim, Tae Gwan | * |
dc.author.google | Lee, Eun-Hee | * |
dc.author.google | Cho, Kyung-Suk | * |
dc.contributor.scopusid | 조경숙(7403957095) | * |
dc.contributor.scopusid | 김태관(57211805072;55151157100) | * |
dc.contributor.scopusid | 이은희(55773710100;56141882500) | * |
dc.date.modifydate | 20240322131338 | * |