참깨유와 들깨유를 급원으로w-6와 w-3지방산 비율을 달리한 식이가 휜쥐의 혈청 지질함량과 혈소판 기능에 미치는 영향

Abstract

(이전원문누락) 5. 혈소판 TXA_(2) 생성량의 지표인 malondialdehyde(MDA) 생성량은 linoleic acid에 대한 linolenic acid의 비율이 증가할 수록 감소하였고 D0/0군이 다른 군에 비해 유의적으로 높았다. 그러나 사육기간에 따른 유의적인 차이는 없었다. 6. Linoleic acid/linolenic acid의 비율을 달리한 여러 식이와 사육기간이 혈소판의 지방산 조성에 미치는 영향을 보면 식이의 linolenic acid 함량이 증가할 수록 eicosapentaenoic acid(EPA,20:5,ω-3)/arachidonic acid(AA,20:4,ω-6)의 비율이 점차 증가하였다. 이러한 EPA/AA 비율의 증가는 혈소판에서 생성되는 MDA 양의 감소와 그에 따른 출혈시간과 전혈용고시간의 연장을 설명해준다. 결론적으로 본 실험은 식이지방의 linolenic acid함량을 증가시켰을 때 혈청지질함량이 감소하는 효과(hypolipidemic effect)를 보여주었다. 또한 linolenic acid로 부터 EPA로 전환의 증가는 혈소판내의 EPA/AA를 증가시키며, 혈전 경향을 감소시켜서 출혈시간과 전혈용고시간을 지연시킴을 확인하였다.;In this experiment, we investigated the hypolipidemic and antithrombotic effects of rats fed diets with different linoleic to linolenic acid ratios for different feeding periods(4 or 8 months), using Korean sesame oil as the source of linoleic acid(18:2, ω-6) and perilla oil as the source of linolenic acid(18:3, ω-3). The result were as follows: 1. Comparing DO/O to D4/20, Serum lipids(total lipids, triglyceride, total cholesterol, HDL-cholesterol) were decreased in augumented linolenic acid intakes. (hypolipidemic effect) 2. Malondialdehyde(MDA) was determined in place of thromboxane A_(2)(TXA_(2)) in Platelets. The level of MDA generation during thrombin-induced aggregation of platelets was decreased with augumented linolenic acid intakes. This result can suggest that linolenic acid seem to suppress the conversion of linoleic acid to arachidonic acid(AA, 20:4, ω-6) and eicosapentaenoic acid(EPA, 20:5, ω-3) transformed from linolenic acid to suppress the conversion of arachidonic acid to TXA_(2). 3. With regard to the composition of platelet fatty acids, the ratio of EPA to Ah was increased gradually with increased linolenic acid intakes, indicating a chain elongation and desaturation of linolenic acid to EPA, with slight exception of animals fed D4/8. It can be controversial, but suggest that there is ideal mixing ratio of ω-6 and ω-3 in diet 4. Increment of linolenic acid intakes decreased bleeding time and whole blood time: this results from the suppressed conversion of linoleic acid to arachidonic acid and arachidonic acid to TXA_(2) with increased linolenic acid intakes. Since TXA_(2) is platelet-aggregating and vasoconstricting agent, the reduction of TXA_(2) generation by platelets with increased linolenic acid intakes is shown to act as antithrombotic effects. 5. In conclusion, it is noteworthy that perilla oil containing lots of linolenic acid is hypolipidemic and antithrombotic effects.