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K-ras mutation detected by peptide nucleic acid-clamping polymerase chain reaction, Ki-67, S100P, and SMAD4 expression can improve the diagnostic accuracy of inconclusive pancreatic EUS-FNB specimens
- Title
- K-ras mutation detected by peptide nucleic acid-clamping polymerase chain reaction, Ki-67, S100P, and SMAD4 expression can improve the diagnostic accuracy of inconclusive pancreatic EUS-FNB specimens
- Authors
- Shin; Eun; Kim; Bo-Hyung; Kwon; Minji; Lee; Donghwan; Park; Se Woo
- Ewha Authors
- 이동환
- SCOPUS Author ID
- 이동환
- Issue Date
- 2024
- Journal Title
- Pancreatology
- ISSN
- 1424-3903
- Citation
- Pancreatology vol. 24, no. 4, pp. 584 - 591
- Keywords
- Immunohistochemistry; K-ras; Pancreatic carcinoma; PNA-Clamping; Sono-guided biopsy
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Objectives: We aimed to assess the diagnostic utility of an immunohistochemical panel including calcium-binding protein P, p53, Ki-67, and SMAD family member 4 and K-ras mutation for diagnosing pancreatic solid lesion specimens obtained by endoscopic ultrasound-guided fine-needle biopsy and to confirm their usefulness in histologically inconclusive cases. Methods: Immunohistochemistry and peptide nucleic acid-clamping polymerase chain reaction for K-ras mutation were performed on 96 endoscopic ultrasound-guided fine-needle biopsy specimens. The diagnostic efficacy of each marker and the combination of markers was calculated. The diagnostic performances of these markers were evaluated in 27 endoscopic ultrasound-guided fine-needle biopsy specimens with histologically inconclusive diagnoses. A classification tree was constructed. Results: K-ras mutation showed the highest accuracy and consistency. Positivity in more than two or three of the five markers showed high diagnostic accuracy (94.6 % and 93.6 %, respectively), and positivity for more than three markers showed the highest accuracy for inconclusive cases (92.0 %). A classification tree using K-ras mutation, Ki-67, S100P, and SMAD4 showed high diagnostic performance, with only two misclassifications in inconclusive cases. Conclusions: K-ras mutation detection via peptide nucleic acid-clamping polymerase chain reaction is a stable and accurate method for distinguishing between pancreatic ductal adenocarcinoma and non-pancreatic ductal adenocarcinoma lesions. A classification tree using K-ras mutation, Ki-67, S100P, and SMAD4 helps increase the diagnostic accuracy of cases that are histologically difficult to diagnose. © 2024 IAP and EPC
- DOI
- 10.1016/j.pan.2024.04.005
- Appears in Collections:
- 자연과학대학 > 통계학전공 > Journal papers
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