Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이동환 | - |
dc.date.accessioned | 2024-08-23T16:30:46Z | - |
dc.date.available | 2024-08-23T16:30:46Z | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 1424-3903 | - |
dc.identifier.other | OAK-35872 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/269270 | - |
dc.description.abstract | Objectives: We aimed to assess the diagnostic utility of an immunohistochemical panel including calcium-binding protein P, p53, Ki-67, and SMAD family member 4 and K-ras mutation for diagnosing pancreatic solid lesion specimens obtained by endoscopic ultrasound-guided fine-needle biopsy and to confirm their usefulness in histologically inconclusive cases. Methods: Immunohistochemistry and peptide nucleic acid-clamping polymerase chain reaction for K-ras mutation were performed on 96 endoscopic ultrasound-guided fine-needle biopsy specimens. The diagnostic efficacy of each marker and the combination of markers was calculated. The diagnostic performances of these markers were evaluated in 27 endoscopic ultrasound-guided fine-needle biopsy specimens with histologically inconclusive diagnoses. A classification tree was constructed. Results: K-ras mutation showed the highest accuracy and consistency. Positivity in more than two or three of the five markers showed high diagnostic accuracy (94.6 % and 93.6 %, respectively), and positivity for more than three markers showed the highest accuracy for inconclusive cases (92.0 %). A classification tree using K-ras mutation, Ki-67, S100P, and SMAD4 showed high diagnostic performance, with only two misclassifications in inconclusive cases. Conclusions: K-ras mutation detection via peptide nucleic acid-clamping polymerase chain reaction is a stable and accurate method for distinguishing between pancreatic ductal adenocarcinoma and non-pancreatic ductal adenocarcinoma lesions. A classification tree using K-ras mutation, Ki-67, S100P, and SMAD4 helps increase the diagnostic accuracy of cases that are histologically difficult to diagnose. © 2024 IAP and EPC | - |
dc.description.sponsorship | Elsevier B.V. | - |
dc.language | English | - |
dc.subject | Immunohistochemistry | - |
dc.subject | K-ras | - |
dc.subject | Pancreatic carcinoma | - |
dc.subject | PNA-Clamping | - |
dc.subject | Sono-guided biopsy | - |
dc.title | K-ras mutation detected by peptide nucleic acid-clamping polymerase chain reaction, Ki-67, S100P, and SMAD4 expression can improve the diagnostic accuracy of inconclusive pancreatic EUS-FNB specimens | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 24 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 584 | - |
dc.relation.lastpage | 591 | - |
dc.relation.journaltitle | Pancreatology | - |
dc.identifier.doi | 10.1016/j.pan.2024.04.005 | - |
dc.identifier.scopusid | 2-s2.0-85191879799 | - |
dc.author.google | Shin | - |
dc.author.google | Eun | - |
dc.author.google | Kim | - |
dc.author.google | Bo-Hyung | - |
dc.author.google | Kwon | - |
dc.author.google | Minji | - |
dc.author.google | Lee | - |
dc.author.google | Donghwan | - |
dc.author.google | Park | - |
dc.author.google | Se Woo | - |
dc.contributor.scopusid | 이동환(56434427300;58539708000) | - |
dc.date.modifydate | 20240823162314 | - |