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Anti-Inflammatory Activity of the Constituents from the Leaves of Perilla frutescens var. acuta
- Title
- Anti-Inflammatory Activity of the Constituents from the Leaves of Perilla frutescens var. acuta
- Authors
- Youn; Isoo; Han; Sujin; Jung; Hee Jin; Noh; Sang Gyun; Chung; Hae Young; Koo; Yean Kyoung; Shin; Sunhye; Seo; Eun Kyoung
- Ewha Authors
- 서은경; 윤이수
- SCOPUS Author ID
- 서은경; 윤이수
- Issue Date
- 2023
- Journal Title
- Pharmaceuticals
- ISSN
- 1424-8247
- Citation
- Pharmaceuticals vol. 16, no. 12
- Keywords
- anti-inflammation; flavonoid diglucuronide; nuclear factor kappa B; Perilla frutescens var. acuta; peroxisome proliferator-activated receptor
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Perilla frutense var. acuta (Lamiaceae) has been used to treat indigestion, asthma, and allergies in traditional medicine. In this study, luteolin 7-O-diglucuronide (1), apigenin 7-O-diglucuronide (2), and rosmarinic acid (3) were isolated from the leaves of P. frutescens var. acuta through various chromatographic purification techniques. Several approaches were used to investigate the anti-inflammatory activity of the constituents (1–3) and their working mechanisms. In silico docking simulation demonstrated that 1–3 would work as a PPAR-α/δ/γ agonist, and in vitro PPAR-α/δ/γ transcriptional assay showed that the Perilla water extract (PWE) and 3 increased PPAR-α luciferase activity (1.71 and 1.61 times of the control (PPAR-α + PPRE, p < 0.001)). In the NF-κB luciferase assay, 1 suppressed NF-κB activity the most (56.83% at 5 µM; 74.96% at 10 µM; 79.86% at 50 µM). In addition, 1 and 2 inhibited the mRNA expression of NF-κB target genes, including Il6, Mcp1, and Tnfa, at 50 µM, and 3 suppressed the genes at the mRNA level in a dose-dependent manner. We report that 1 and 2 exert anti-inflammatory effects through NF-κB inhibition, and the PPAR-α/NF-κB signaling pathway is related to the anti-inflammatory activity of 3. © 2023 by the authors.
- DOI
- 10.3390/ph16121655
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
- Files in This Item:
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pharmaceuticals-16-01655.pdf(2.16 MB)
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