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Development of fluorescence-linked immunosorbent assay for rapid detection of Staphylococcus aureus
- Title
- Development of fluorescence-linked immunosorbent assay for rapid detection of Staphylococcus aureus
- Authors
- Kim; Joo-Kyung; Yun; Hyun-Young; Jae-Seok; Wooseong; Lee; Chang-Soo; Byung-Gee; Jeong; Hee-Jin
- Ewha Authors
- 김우성
- SCOPUS Author ID
- 김우성
- Issue Date
- 2024
- Journal Title
- Applied Microbiology and Biotechnology
- ISSN
- 0175-7598
- Citation
- Applied Microbiology and Biotechnology vol. 108, no. 1, pp. 1 - 10
- Keywords
- ELISA; FLISA; Fluorescent antibody; Immunoassay; Staphylococcus aureus
- Publisher
- Springer Science and Business Media Deutschland GmbH
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Abstract: Staphylococcus aureus is a major pathogen that causes infections and life-threatening diseases. Although antibiotics, such as methicillin, have been used, methicillin-resistant S. aureus (MRSA) causes high morbidity and mortality rates, and conventional detection methods are difficult to be used because of time-consuming process. To control the spread of S. aureus, a development of a rapid and simple detection method is required. In this study, we generated a fluorescent anti-S. aureus antibody, and established a novel fluorescence-linked immunosorbent assay (FLISA)-based S. aureus detection method. The method showed high sensitivity and low limit of detection toward MRSA detection. The assay time for FLISA was 5 h, which was faster than that of conventional enzyme-linked immunosorbent assay (ELISA) or rapid ELISA. Moreover, the FLISA-based detection method was applied to diagnose clinically isolated MRSA samples that required only 5.3 h of preincubation. The FLISA method developed in this study can be widely applied as a useful tool for convenient S. aureus detection. Key points: • A fluorescence-linked immunosorbent assay-based S. aureus detection method • Simultaneous quantification of a maximum of 96 samples within 5 h • Application of the novel system to diagnosis clinical isolates Graphical Abstract: [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
- DOI
- 10.1007/s00253-023-12836-2
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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