Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김우성 | * |
dc.date.accessioned | 2024-02-06T16:31:15Z | - |
dc.date.available | 2024-02-06T16:31:15Z | - |
dc.date.issued | 2024 | * |
dc.identifier.issn | 0175-7598 | * |
dc.identifier.other | OAK-34529 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/267042 | - |
dc.description.abstract | Abstract: Staphylococcus aureus is a major pathogen that causes infections and life-threatening diseases. Although antibiotics, such as methicillin, have been used, methicillin-resistant S. aureus (MRSA) causes high morbidity and mortality rates, and conventional detection methods are difficult to be used because of time-consuming process. To control the spread of S. aureus, a development of a rapid and simple detection method is required. In this study, we generated a fluorescent anti-S. aureus antibody, and established a novel fluorescence-linked immunosorbent assay (FLISA)-based S. aureus detection method. The method showed high sensitivity and low limit of detection toward MRSA detection. The assay time for FLISA was 5 h, which was faster than that of conventional enzyme-linked immunosorbent assay (ELISA) or rapid ELISA. Moreover, the FLISA-based detection method was applied to diagnose clinically isolated MRSA samples that required only 5.3 h of preincubation. The FLISA method developed in this study can be widely applied as a useful tool for convenient S. aureus detection. Key points: • A fluorescence-linked immunosorbent assay-based S. aureus detection method • Simultaneous quantification of a maximum of 96 samples within 5 h • Application of the novel system to diagnosis clinical isolates Graphical Abstract: [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. | * |
dc.language | English | * |
dc.publisher | Springer Science and Business Media Deutschland GmbH | * |
dc.subject | ELISA | * |
dc.subject | FLISA | * |
dc.subject | Fluorescent antibody | * |
dc.subject | Immunoassay | * |
dc.subject | Staphylococcus aureus | * |
dc.title | Development of fluorescence-linked immunosorbent assay for rapid detection of Staphylococcus aureus | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 108 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1 | * |
dc.relation.lastpage | 10 | * |
dc.relation.journaltitle | Applied Microbiology and Biotechnology | * |
dc.identifier.doi | 10.1007/s00253-023-12836-2 | * |
dc.identifier.wosid | WOS:001132530600001 | * |
dc.identifier.scopusid | 2-s2.0-85180710127 | * |
dc.author.google | Kim | * |
dc.author.google | Joo-Kyung | * |
dc.author.google | Yun | * |
dc.author.google | Hyun-Young | * |
dc.author.google | Jae-Seok | * |
dc.author.google | Wooseong | * |
dc.author.google | Lee | * |
dc.author.google | Chang-Soo | * |
dc.author.google | Byung-Gee | * |
dc.author.google | Jeong | * |
dc.author.google | Hee-Jin | * |
dc.contributor.scopusid | 김우성(57201881427) | * |
dc.date.modifydate | 20240502145036 | * |