Ni-rGO Sensor Combined with Human Olfactory Receptor-Embedded Nanodiscs for Detecting Gas-Phase DMMP as a Simulant of Nerve Agents

Abstract

Nerve agents are organophosphorus toxic chemicals that can inhibit acetylcholinesterase, leading to paralysis of the nervous system and death. Early detection of nerve agents is important for safety issues. Dimethyl methylphosphonate (DMMP) is widely used as a simulant of nerve agents, and many studies have been conducted using DMMP as a substitute for detecting nerve agents. Despite many studies on sensors for detecting DMMP, they have limitations in sensitivity and selectivity. To overcome these limitations, a nickel-decorated reduced graphene oxide (Ni-rGO) sensor with human olfactory receptor hOR2T7 nanodiscs was utilized to create a bioelectronic nose platform for DMMP gas detection. hOR2T7 was produced and reconstituted into nanodiscs for enhancing the sensor’s stability, especially for detection in a gas phase. It could detect DMMP gas selectively and repeatedly at a concentration of 1 ppb. This sensitive and selective bioelectronic nose can be applied as a practical tool for the detection of gaseous chemical warfare agents in military and safety fields. © 2023 American Chemical Society