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Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2
- Title
- Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2
- Authors
- Kim, Ji Woong; Min, Sung Won; Lee, Jichul; Shin, Ha Gyeong; Choi, Hye Lim; Yang, Ha Rim; Lee, Ji Hyun; Cho, Yea Bin; Shim, Hyunbo; Lee, Sukmook
- Ewha Authors
- 심현보
- SCOPUS Author ID
- 심현보
- Issue Date
- 2022
- Journal Title
- BIOMEDICINES
- ISSN
- 2227-9059
- Citation
- BIOMEDICINES vol. 10, no. 12
- Keywords
- SARS-CoV-2; human antibody; neutralization; sandwich ELISA
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in an ongoing global pandemic crisis, caused by the life-threatening illness coronavirus disease 2019 (COVID-19). Thus, the rapid development of monoclonal antibodies (mAbs) to cope with COVID-19 is urgently necessary. In this study, we used phage display to develop four human mAbs specific to the receptor-binding domain (RBD) of SARS-CoV-2. Our intensive in vitro functional analyses demonstrated that K102.1, an anti-SARS-CoV-2 RBD-specific mAb, exerted potent neutralizing activity against pseudoviral and live viral infection and the interaction between SARS-CoV-2 RBD and human angiotensin-converting enzyme 2. Monotherapy with K102.1 also revealed the therapeutic potential against SARS-CoV-2 infection in vivo. Further, this study developed a sandwich enzyme-linked immunosorbent assay with a non-competing mAb pair, K102.1 and K102.2, that accurately detected the RBDs of SARS-CoV-2 wild-type and variants with high sensitivity in the picomolar range. These findings suggest that the phage-display-based mAb selection from an established antibody library may be an effective strategy for the rapid development of mAbs against the constantly evolving SARS-CoV-2.
- DOI
- 10.3390/biomedicines10123274
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
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biomedicines-10-03274-v2.pdf(3.63 MB)
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