Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 심현보 | * |
dc.date.accessioned | 2023-01-06T16:30:07Z | - |
dc.date.available | 2023-01-06T16:30:07Z | - |
dc.date.issued | 2022 | * |
dc.identifier.issn | 2227-9059 | * |
dc.identifier.other | OAK-32807 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/263061 | - |
dc.description.abstract | Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in an ongoing global pandemic crisis, caused by the life-threatening illness coronavirus disease 2019 (COVID-19). Thus, the rapid development of monoclonal antibodies (mAbs) to cope with COVID-19 is urgently necessary. In this study, we used phage display to develop four human mAbs specific to the receptor-binding domain (RBD) of SARS-CoV-2. Our intensive in vitro functional analyses demonstrated that K102.1, an anti-SARS-CoV-2 RBD-specific mAb, exerted potent neutralizing activity against pseudoviral and live viral infection and the interaction between SARS-CoV-2 RBD and human angiotensin-converting enzyme 2. Monotherapy with K102.1 also revealed the therapeutic potential against SARS-CoV-2 infection in vivo. Further, this study developed a sandwich enzyme-linked immunosorbent assay with a non-competing mAb pair, K102.1 and K102.2, that accurately detected the RBDs of SARS-CoV-2 wild-type and variants with high sensitivity in the picomolar range. These findings suggest that the phage-display-based mAb selection from an established antibody library may be an effective strategy for the rapid development of mAbs against the constantly evolving SARS-CoV-2. | * |
dc.language | English | * |
dc.publisher | MDPI | * |
dc.subject | SARS-CoV-2 | * |
dc.subject | human antibody | * |
dc.subject | neutralization | * |
dc.subject | sandwich ELISA | * |
dc.title | Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2 | * |
dc.type | Article | * |
dc.relation.issue | 12 | * |
dc.relation.volume | 10 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | BIOMEDICINES | * |
dc.identifier.doi | 10.3390/biomedicines10123274 | * |
dc.identifier.wosid | WOS:000902367700001 | * |
dc.identifier.scopusid | 2-s2.0-85144654894 | * |
dc.author.google | Kim, Ji Woong | * |
dc.author.google | Min, Sung Won | * |
dc.author.google | Lee, Jichul | * |
dc.author.google | Shin, Ha Gyeong | * |
dc.author.google | Choi, Hye Lim | * |
dc.author.google | Yang, Ha Rim | * |
dc.author.google | Lee, Ji Hyun | * |
dc.author.google | Cho, Yea Bin | * |
dc.author.google | Shim, Hyunbo | * |
dc.author.google | Lee, Sukmook | * |
dc.contributor.scopusid | 심현보(26635827900) | * |
dc.date.modifydate | 20240123110611 | * |