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C-4-dicarboxylate metabolons: interaction of C-4-dicarboxylate transporters of Escherichia coli with cytosolic enzymes
- Title
- C-4-dicarboxylate metabolons: interaction of C-4-dicarboxylate transporters of Escherichia coli with cytosolic enzymes
- Authors
- Schubert, Christopher; Kim, Nam Yeun; Unden, Gottfried; Bin Kim, Ok
- Ewha Authors
- 김옥빈; 김남연
- SCOPUS Author ID
- 김옥빈
- Issue Date
- 2022
- Journal Title
- FEMS MICROBIOLOGY LETTERS
- ISSN
- 0378-1097
1574-6968
- Citation
- FEMS MICROBIOLOGY LETTERS vol. 369, no. 1
- Keywords
- metabolon; C-4-dicarboxylate transporter; L-aspartate; Escherichia coli; BACTH; mSPINE
- Publisher
- OXFORD UNIV PRESS
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Metabolons represent the structural organization of proteins for metabolic or regulatory pathways. Here, the interaction of fumarase FumB, aspartase AspA, and L-tartrate dehydratase TtdAB with the C4-dicarboxylate (C4-DC) transporters DcuA, DcuB, DcuC, and the L-tartrate transporter TtdT of Escherichia coli was tested by a bacterial two-hybrid (BACTH) assay in situ, or by co-chromatography using mSPINE (membrane Streptavidin protein interaction experiment). From the general C4-DC transporters, DcuB interacted with FumB and AspA, DcuA with AspA, whereas DcuC interacted with neither FumB nor AspA. Moreover, TtdT did not interact with TtdAB. The fumB-dcuB, the dcuA-aspA, and the ttdAB-ttdT genes encoding the respective proteins colocalize on the genome and each pair of genes forms cotranscripts, whereas the dcuC gene lies alone. The data suggest the formation of DcuB/FumB and DcuB/AspA metabolons for the uptake of L-malate, or L-aspartate, and their conversion to fumarate for fumarate respiration and excretion of the product succinate. The DcuA/AspA metabolon catalyzes uptake and conversion of L-aspartate to fumarate coupled to succinate excretion. The DcuA/AspA metabolon provides ammonia at the same time for nitrogen assimilation (ammonia shuttle). On the other hand, TtdT and TtdAB are not organized in a metabolon. Reasons for the formation (DcuA/AspA, DcuB/FumB, and DcuB/AspA) or nonformation (DcuC, TtdT, and TtdAB) of metabolons are discussed based on their metabolic roles. Experimental measurement for spatial proximity revealed that transporters for general C-4-dicarboxylates, such as L-malate and L-aspartate, form metabolons with cytosolic enzymes, however, no metabolons were observed for noncommon C-4-dicarboxylates.
- DOI
- 10.1093/femsle/fnac078
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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