Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김옥빈 | * |
dc.contributor.author | 김남연 | * |
dc.date.accessioned | 2022-10-27T16:31:15Z | - |
dc.date.available | 2022-10-27T16:31:15Z | - |
dc.date.issued | 2022 | * |
dc.identifier.issn | 0378-1097 | * |
dc.identifier.issn | 1574-6968 | * |
dc.identifier.other | OAK-32337 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/262679 | - |
dc.description.abstract | Metabolons represent the structural organization of proteins for metabolic or regulatory pathways. Here, the interaction of fumarase FumB, aspartase AspA, and L-tartrate dehydratase TtdAB with the C4-dicarboxylate (C4-DC) transporters DcuA, DcuB, DcuC, and the L-tartrate transporter TtdT of Escherichia coli was tested by a bacterial two-hybrid (BACTH) assay in situ, or by co-chromatography using mSPINE (membrane Streptavidin protein interaction experiment). From the general C4-DC transporters, DcuB interacted with FumB and AspA, DcuA with AspA, whereas DcuC interacted with neither FumB nor AspA. Moreover, TtdT did not interact with TtdAB. The fumB-dcuB, the dcuA-aspA, and the ttdAB-ttdT genes encoding the respective proteins colocalize on the genome and each pair of genes forms cotranscripts, whereas the dcuC gene lies alone. The data suggest the formation of DcuB/FumB and DcuB/AspA metabolons for the uptake of L-malate, or L-aspartate, and their conversion to fumarate for fumarate respiration and excretion of the product succinate. The DcuA/AspA metabolon catalyzes uptake and conversion of L-aspartate to fumarate coupled to succinate excretion. The DcuA/AspA metabolon provides ammonia at the same time for nitrogen assimilation (ammonia shuttle). On the other hand, TtdT and TtdAB are not organized in a metabolon. Reasons for the formation (DcuA/AspA, DcuB/FumB, and DcuB/AspA) or nonformation (DcuC, TtdT, and TtdAB) of metabolons are discussed based on their metabolic roles. Experimental measurement for spatial proximity revealed that transporters for general C-4-dicarboxylates, such as L-malate and L-aspartate, form metabolons with cytosolic enzymes, however, no metabolons were observed for noncommon C-4-dicarboxylates. | * |
dc.language | English | * |
dc.publisher | OXFORD UNIV PRESS | * |
dc.subject | metabolon | * |
dc.subject | C-4-dicarboxylate transporter | * |
dc.subject | L-aspartate | * |
dc.subject | Escherichia coli | * |
dc.subject | BACTH | * |
dc.subject | mSPINE | * |
dc.title | C-4-dicarboxylate metabolons: interaction of C-4-dicarboxylate transporters of Escherichia coli with cytosolic enzymes | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 369 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | FEMS MICROBIOLOGY LETTERS | * |
dc.identifier.doi | 10.1093/femsle/fnac078 | * |
dc.identifier.wosid | WOS:000856344400002 | * |
dc.identifier.scopusid | 2-s2.0-85138491962 | * |
dc.author.google | Schubert, Christopher | * |
dc.author.google | Kim, Nam Yeun | * |
dc.author.google | Unden, Gottfried | * |
dc.author.google | Bin Kim, Ok | * |
dc.contributor.scopusid | 김옥빈(15851079900) | * |
dc.date.modifydate | 20240301081003 | * |