View : 343 Download: 0
Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
- Title
- Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
- Authors
- Lim, Su-Jin; Min, Dae-Jin; Kim, Sohee; Lee, Jihye; Lee, Eun-Soo; Kim, Hyuk; Cho, Sung-Yoen; Beak, Heung-Soo; Lee, Chang-Seok; Nam, Sang-Jip; Ko, Jaeyoung
- Ewha Authors
- 남상집
- SCOPUS Author ID
- 남상집
- Issue Date
- 2021
- Journal Title
- MARINE DRUGS
- ISSN
- 1660-3397
- Citation
- MARINE DRUGS vol. 19, no. 11
- Keywords
- <p>Pseudoalteromonas sp.</p>; pseudoalteromone A; marine natural product; melanogenesis
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- An ubiquinone derivative, pseudoalteromone A (1), has been isolated from two marine-derived Pseudoalteromonas spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and extracellular melanin content and cellular tyrosinase activity in the B16 cell line, Melan-a mouse melanocyte cell line, and MNT-1 human malignant melanoma cell line. Treatment with pseudoalteromone A (40 mu g/mL) for 72 h reduced alpha-melanocyte-stimulating hormone (alpha-MSH)-induced intracellular melanin production by up to 44.68% in B16 cells and 38.24% in MNT-1 cells. Notably, pseudoalteromone A induced a concentration-dependent reduction in cellular tyrosinase activity in B16 cell, and Western blot analyses showed that this inhibitory activity was associated with a significant decrease in protein levels of tyrosinase and tyrosinase-related protein 1 (Tyrp-1), suggesting that pseudoalteromone A exerts its anti-melanogenesis activity through effects on melanogenic genes. We further evaluated the skin-whitening effect of pseudoalteromone A in the three-dimensional (3D) pigmented-epidermis model, MelanoDerm, and visualized the 3D distribution of melanin by two-photon excited fluorescence imaging in this human skin equivalent. Collectively, our findings suggest that pseudoalteromone A inhibits tyrosinase activity and expression and that this accounts for its anti-melanogenic effects in melanocytes.
- DOI
- 10.3390/md19110612
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML