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Reasonably constructed NIR fluorescent probes based on dicyanoisophorone skeleton for imaging ONOO- in living cells
- Title
- Reasonably constructed NIR fluorescent probes based on dicyanoisophorone skeleton for imaging ONOO- in living cells
- Authors
- Chung, Jeewon; Kim, Heejeong; Li, Haidong; Yoon, Juyoung
- Ewha Authors
- 윤주영
- SCOPUS Author ID
- 윤주영
- Issue Date
- 2021
- Journal Title
- DYES AND PIGMENTS
- ISSN
- 0143-7208
1873-3743
- Citation
- DYES AND PIGMENTS vol. 195
- Keywords
- Reactive nitrogen species; pKa regulation; Cell imaging; NIR Fluorescent probe
- Publisher
- ELSEVIER SCI LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Peroxynitrite (ONOO-), a reactive nitrogen species (RNS), engages in various cellular processes including inflammation, apoptosis, and necrosis. Therefore, the development of a powerful tool for imaging endogenous ONOO- is important with regard to revealing its underlying physiological or pathological functions. In this work, we introduced dihalogen (Cl, Br, and I) at the ortho-positions of the hydroxyl group of a dicyanoisophorone skeleton to adjust its pKa to be more suitable for bio-imaging in the physiological environments with a high signal-to-noise ratio. Based on the optimized spectral results and the requirement of biocompatibility, the fluorescent probe NIR-dCl was successfully designed and fabricated for the detection of ONOO-. Upon the addition of ONOO- in solution, an obvious NIR fluorescent signal at 680 nm was observed. Moreover, the probe NIR-dCl exhibited high selectivity for sensing ONOO-. Cell imaging results confirmed that probe NIR-dCl was capable of imaging exogenous and endogenous ONOO- in live cells. Thus, we believe that probe NIR-dCl could be an effective tool for studying biochemical processes and ONOO--related diseases.
- DOI
- 10.1016/j.dyepig.2021.109665
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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