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Fluorescence Probe for Imaging N-Methyl-D-aspartate Receptors and Monitoring GSH Selectively Using Two-Photon Microscopy
- Title
- Fluorescence Probe for Imaging N-Methyl-D-aspartate Receptors and Monitoring GSH Selectively Using Two-Photon Microscopy
- Authors
- Kwon, Nahyun; Lim, Chang Su; Ko, Gyeongju; Ha, Jeongsun; Lee, Dayoung; Yin, Jun; Kim, Hwan Myung; Yoon, Juyoung
- Ewha Authors
- 윤주영
- SCOPUS Author ID
- 윤주영
- Issue Date
- 2021
- Journal Title
- ANALYTICAL CHEMISTRY
- ISSN
- 0003-2700
1520-6882
- Citation
- ANALYTICAL CHEMISTRY vol. 93, no. 33, pp. 11612 - 11616
- Publisher
- AMER CHEMICAL SOC
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- N-Methyl-D-aspartate (NMDA) is an excitotoxic amino acid used to identify a specific subset of glutamate receptors. The activity of NMDA receptors is closely related to the redox level of the biological system. Glutathione (GSH) as an antioxidant plays a key role with regard to modulation of the redox environment. In this work we designed and developed a GSH-specific fluorescent probe with the capability of targeting NMDA receptors, which was composed of a two-photon naphthalimide fluorophore, a GSHreactive group sulfonamide, and an ifenprodil targeting group for the NMDA receptor. This probe exhibited high selectivity toward GSH in comparison to other similar amino acids. Two-photon fluorescence microscopy allowed this probe to successfully monitor GSH in neuronal cells and hippocampal tissues with an excitation at 750 nm. It could serve as a potential practical imaging tool to explore the function of GSH and related biological processes in the brain.
- DOI
- 10.1021/acs.analchem.1c02350
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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