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Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice
- Title
- Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice
- Authors
- Hong, Heejeong; Song, Hyun-Kuk; Jang, Bohee; Park, Eunhye; Han, Dong Soo; Kim, Seong-Eun; Oh, Eok-Soo
- Ewha Authors
- 오억수; 김성은; 장보희
- SCOPUS Author ID
- 오억수; 김성은; 장보희
- Issue Date
- 2021
- Journal Title
- JOURNAL OF INFLAMMATION RESEARCH
- ISSN
- 1178-7031
- Citation
- JOURNAL OF INFLAMMATION RESEARCH vol. 14, pp. 4987 - 5000
- Keywords
- chronic inflammation; syndecan-2; MMP-7; shedding
- Publisher
- DOVE MEDICAL PRESS LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Introduction: Syndecan-2 expression is elevated during chronic inflammation and cancer development, and its shedding is observed in cancer patients. However, it remained unknown whether inflammation triggers syndecan-2 shedding. Methods: The colitis model was produced in C57BL/6 mice by oral administration of 2-3% dextran sulfate sodium (DSS) in the drinking water. Syndecan-2 and MMP-7 expression levels in tissues and cells were detected by real-time PCR, Western blotting, and immunohistochemistry. Shed syndecan-2 levels were detected by slot blotting. For tissue culture, colon tissues were divided into proximal, transverse, and distal parts, and incubated in culture media. Results: In C57BL/6 mice with DSS-induced colitis, syndecan-2 shedding began to increase after week 12 of chronic inflammation and continued to increase at week 15. The level of shed syndecan-2 correlated with the colocalization of syndecan-2 and MMP-7 in distal colon tissues. The mRNA expression of IL-6 was increased specifically in trans-distal colon tissues from weeks 9 to 15. IL-6 induced syndecan-2 expression and shedding and MMP-7 expression in ex vivo-cultured distal colon tissues and adenoma cell lines derived from the distal colon. IL-6 treatment induced STAT3 phosphorylation and MMP-7 expression in DLD-1 cells. The application of MMP-7 to ex vivo-cultured colon tissues increased the shedding of syndecan-2 to the culture medium. Conclusion: Our findings suggest that chronic inflammation induces syndecan-2 shedding via the site-specific colocalization of syndecan-2 with MMP-7 in the distal colon.
- DOI
- 10.2147/JIR.S329234
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
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JIR-329234-colocalization-with-mmp-7-in-the-distal-colon-is-crucial-for.pdf(8.98 MB)
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