Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | * |
dc.contributor.author | 김성은 | * |
dc.contributor.author | 장보희 | * |
dc.date.accessioned | 2021-11-09T16:31:03Z | - |
dc.date.available | 2021-11-09T16:31:03Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 1178-7031 | * |
dc.identifier.other | OAK-30258 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/259222 | - |
dc.description.abstract | Introduction: Syndecan-2 expression is elevated during chronic inflammation and cancer development, and its shedding is observed in cancer patients. However, it remained unknown whether inflammation triggers syndecan-2 shedding. Methods: The colitis model was produced in C57BL/6 mice by oral administration of 2-3% dextran sulfate sodium (DSS) in the drinking water. Syndecan-2 and MMP-7 expression levels in tissues and cells were detected by real-time PCR, Western blotting, and immunohistochemistry. Shed syndecan-2 levels were detected by slot blotting. For tissue culture, colon tissues were divided into proximal, transverse, and distal parts, and incubated in culture media. Results: In C57BL/6 mice with DSS-induced colitis, syndecan-2 shedding began to increase after week 12 of chronic inflammation and continued to increase at week 15. The level of shed syndecan-2 correlated with the colocalization of syndecan-2 and MMP-7 in distal colon tissues. The mRNA expression of IL-6 was increased specifically in trans-distal colon tissues from weeks 9 to 15. IL-6 induced syndecan-2 expression and shedding and MMP-7 expression in ex vivo-cultured distal colon tissues and adenoma cell lines derived from the distal colon. IL-6 treatment induced STAT3 phosphorylation and MMP-7 expression in DLD-1 cells. The application of MMP-7 to ex vivo-cultured colon tissues increased the shedding of syndecan-2 to the culture medium. Conclusion: Our findings suggest that chronic inflammation induces syndecan-2 shedding via the site-specific colocalization of syndecan-2 with MMP-7 in the distal colon. | * |
dc.language | English | * |
dc.publisher | DOVE MEDICAL PRESS LTD | * |
dc.subject | chronic inflammation | * |
dc.subject | syndecan-2 | * |
dc.subject | MMP-7 | * |
dc.subject | shedding | * |
dc.title | Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice | * |
dc.type | Article | * |
dc.relation.volume | 14 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 4987 | * |
dc.relation.lastpage | 5000 | * |
dc.relation.journaltitle | JOURNAL OF INFLAMMATION RESEARCH | * |
dc.identifier.doi | 10.2147/JIR.S329234 | * |
dc.identifier.wosid | WOS:000704287600001 | * |
dc.identifier.scopusid | 2-s2.0-85116463721 | * |
dc.author.google | Hong, Heejeong | * |
dc.author.google | Song, Hyun-Kuk | * |
dc.author.google | Jang, Bohee | * |
dc.author.google | Park, Eunhye | * |
dc.author.google | Han, Dong Soo | * |
dc.author.google | Kim, Seong-Eun | * |
dc.author.google | Oh, Eok-Soo | * |
dc.contributor.scopusid | 오억수(7101967153) | * |
dc.contributor.scopusid | 김성은(35210756100;57204243828) | * |
dc.contributor.scopusid | 장보희(55242403100) | * |
dc.date.modifydate | 20240422125929 | * |