View : 472 Download: 0
Enhancing acid tolerance of Escherichia coli via viroporin-mediated export of protons and its application for efficient whole-cell biotransformation
- Title
- Enhancing acid tolerance of Escherichia coli via viroporin-mediated export of protons and its application for efficient whole-cell biotransformation
- Authors
- Shin, Jonghyeok; Jin, Yong-Su; Park, Yong-Cheol; Park, Jin-Byung; Lee, Young-Oh; Kim, Sun-Ki; Kweon, Dae-Hyuk
- Ewha Authors
- 박진병
- SCOPUS Author ID
- 박진병
- Issue Date
- 2021
- Journal Title
- METABOLIC ENGINEERING
- ISSN
- 1096-7176
1096-7184
- Citation
- METABOLIC ENGINEERING vol. 67, pp. 277 - 284
- Keywords
- Influenza A matrix-2 protein; Acid tolerance; Whole-cell biotransformation; (Z)-11-(heptanolyoxy)undec-9-enoic acid; 2 '-Fucosyllactose
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Escherichia coli-based whole-cell biocatalysts are widely used for the sustainable production of value-added chemicals. However, weak acids present as substrates and/or products obstruct the growth and fermentation capability of E. coli. Here, we show that a viroporin consisting of the influenza A matrix-2 (M2) protein, is activated by low pH and has proton channel activity in E. coli. The heterologous expression of the M2 protein in E. coli resulted in a significant increase in the intracellular pH and cell viability in the presence of various weak acids with different lengths of carbon chains. In addition, the feasibility of developing a robust and efficient E. coli-based whole-cell biocatalyst via introduction of the proton-selective viroporin was explored by employing (Z)-11-(heptanolyoxy)undec-9-enoic acid (ester) and 2-fucosyllactose (2'-FL) as model products, whose production is hampered by cytosolic acidification. The engineered E. coli strains containing the proton-selective viroporin exhibited approximately 80% and 230% higher concentrations of the ester and 2'-FL, respectively, than the control strains without the M2 protein. The simple and powerful strategy developed in this study can be applied to produce other valuable chemicals whose production involves substrates and/or products that cause cytosolic acidification.
- DOI
- 10.1016/j.ymben.2021.07.007
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML