Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박진병 | * |
dc.date.accessioned | 2021-08-12T16:32:30Z | - |
dc.date.available | 2021-08-12T16:32:30Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 1096-7176 | * |
dc.identifier.issn | 1096-7184 | * |
dc.identifier.other | OAK-29849 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/258817 | - |
dc.description.abstract | Escherichia coli-based whole-cell biocatalysts are widely used for the sustainable production of value-added chemicals. However, weak acids present as substrates and/or products obstruct the growth and fermentation capability of E. coli. Here, we show that a viroporin consisting of the influenza A matrix-2 (M2) protein, is activated by low pH and has proton channel activity in E. coli. The heterologous expression of the M2 protein in E. coli resulted in a significant increase in the intracellular pH and cell viability in the presence of various weak acids with different lengths of carbon chains. In addition, the feasibility of developing a robust and efficient E. coli-based whole-cell biocatalyst via introduction of the proton-selective viroporin was explored by employing (Z)-11-(heptanolyoxy)undec-9-enoic acid (ester) and 2-fucosyllactose (2'-FL) as model products, whose production is hampered by cytosolic acidification. The engineered E. coli strains containing the proton-selective viroporin exhibited approximately 80% and 230% higher concentrations of the ester and 2'-FL, respectively, than the control strains without the M2 protein. The simple and powerful strategy developed in this study can be applied to produce other valuable chemicals whose production involves substrates and/or products that cause cytosolic acidification. | * |
dc.language | English | * |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | * |
dc.subject | Influenza A matrix-2 protein | * |
dc.subject | Acid tolerance | * |
dc.subject | Whole-cell biotransformation | * |
dc.subject | (Z)-11-(heptanolyoxy)undec-9-enoic acid | * |
dc.subject | 2 '-Fucosyllactose | * |
dc.title | Enhancing acid tolerance of Escherichia coli via viroporin-mediated export of protons and its application for efficient whole-cell biotransformation | * |
dc.type | Article | * |
dc.relation.volume | 67 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 277 | * |
dc.relation.lastpage | 284 | * |
dc.relation.journaltitle | METABOLIC ENGINEERING | * |
dc.identifier.doi | 10.1016/j.ymben.2021.07.007 | * |
dc.identifier.wosid | WOS:000696710500007 | * |
dc.identifier.scopusid | 2-s2.0-85110445584 | * |
dc.author.google | Shin, Jonghyeok | * |
dc.author.google | Jin, Yong-Su | * |
dc.author.google | Park, Yong-Cheol | * |
dc.author.google | Park, Jin-Byung | * |
dc.author.google | Lee, Young-Oh | * |
dc.author.google | Kim, Sun-Ki | * |
dc.author.google | Kweon, Dae-Hyuk | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.date.modifydate | 20240322114808 | * |