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Single-stranded DNA probe paired aptasensor with extra dye binding sites to enhance its fluorescence response in the presence of a target compound
- Title
- Single-stranded DNA probe paired aptasensor with extra dye binding sites to enhance its fluorescence response in the presence of a target compound
- Authors
- Cho, Seo Won; Lim, Hyun Jeong; Chua, Beelee; Son, Ahjeong
- Ewha Authors
- 손아정
- SCOPUS Author ID
- 손아정
- Issue Date
- 2021
- Journal Title
- RSC ADVANCES
- ISSN
- 2046-2069
- Citation
- RSC ADVANCES vol. 11, no. 35, pp. 21796 - 21804
- Publisher
- ROYAL SOC CHEMISTRY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The purpose of this study is to investigate the possibility of improving the performance of a DNA binding dye water quenching based aptasensor without changing or truncating the aptamer. To demonstrate the possibility of increasing the change in fluorescence of the aptasensor by pairing it with a suitable ssDNA probe, three ssDNA probes (probe 1, 2, and 3) were employed and the fluorescence from the bound dyes was measured. This showed that ssDNA probe 2 created the most additional binding sites. By varying the target compound concentration (0, 0.05, 0.5, 5, 50, and 500 mg L-1 4-n-nonylphenol), the corresponding change in the fluorescence signal of the unpaired and ssDNA probe paired aptasensors were measured and compared over a range of emission wavelengths. The response of all three ssDNA probe paired aptasensors showed good fit (R-2 = 0.88-0.92) to a logarithmic response. The sensitivity of the aptasensor paired with ssDNA probe 2 was improved by similar to 60%, whereas that of the aptasensor paired with ssDNA probe 3 was only improved by a marginal similar to 3%. This study is a demonstration of using an appropriate ssDNA probe to increase the number of binding sites and hence the performance of a DNA binding dye and water quenched aptasensor. It is a possibility that can be extended to similar aptasensors without having to change or truncate the aptamer.
- DOI
- 10.1039/d1ra00971k
- Appears in Collections:
- 공과대학 > 환경공학과 > Journal papers
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