Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 손아정 | * |
dc.date.accessioned | 2021-08-05T16:31:23Z | - |
dc.date.available | 2021-08-05T16:31:23Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 2046-2069 | * |
dc.identifier.other | OAK-29611 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/258533 | - |
dc.description.abstract | The purpose of this study is to investigate the possibility of improving the performance of a DNA binding dye water quenching based aptasensor without changing or truncating the aptamer. To demonstrate the possibility of increasing the change in fluorescence of the aptasensor by pairing it with a suitable ssDNA probe, three ssDNA probes (probe 1, 2, and 3) were employed and the fluorescence from the bound dyes was measured. This showed that ssDNA probe 2 created the most additional binding sites. By varying the target compound concentration (0, 0.05, 0.5, 5, 50, and 500 mg L-1 4-n-nonylphenol), the corresponding change in the fluorescence signal of the unpaired and ssDNA probe paired aptasensors were measured and compared over a range of emission wavelengths. The response of all three ssDNA probe paired aptasensors showed good fit (R-2 = 0.88-0.92) to a logarithmic response. The sensitivity of the aptasensor paired with ssDNA probe 2 was improved by similar to 60%, whereas that of the aptasensor paired with ssDNA probe 3 was only improved by a marginal similar to 3%. This study is a demonstration of using an appropriate ssDNA probe to increase the number of binding sites and hence the performance of a DNA binding dye and water quenched aptasensor. It is a possibility that can be extended to similar aptasensors without having to change or truncate the aptamer. | * |
dc.language | English | * |
dc.publisher | ROYAL SOC CHEMISTRY | * |
dc.title | Single-stranded DNA probe paired aptasensor with extra dye binding sites to enhance its fluorescence response in the presence of a target compound | * |
dc.type | Article | * |
dc.relation.issue | 35 | * |
dc.relation.volume | 11 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 21796 | * |
dc.relation.lastpage | 21804 | * |
dc.relation.journaltitle | RSC ADVANCES | * |
dc.identifier.doi | 10.1039/d1ra00971k | * |
dc.identifier.wosid | WOS:000665644000056 | * |
dc.identifier.scopusid | 2-s2.0-85108683047 | * |
dc.author.google | Cho, Seo Won | * |
dc.author.google | Lim, Hyun Jeong | * |
dc.author.google | Chua, Beelee | * |
dc.author.google | Son, Ahjeong | * |
dc.contributor.scopusid | 손아정(23487252600) | * |
dc.date.modifydate | 20240322131724 | * |