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Ozonation enhancement of low cost double-stranded DNA binding dye based fluorescence measurement of total bacterial load in water
- Title
- Ozonation enhancement of low cost double-stranded DNA binding dye based fluorescence measurement of total bacterial load in water
- Authors
- Choi, Jiwon; Chua, Beelee; Son, Ahjeong
- Ewha Authors
- 손아정
- SCOPUS Author ID
- 손아정
- Issue Date
- 2021
- Journal Title
- RSC ADVANCES
- ISSN
- 2046-2069
- Citation
- RSC ADVANCES vol. 11, no. 7, pp. 3931 - 3941
- Publisher
- ROYAL SOC CHEMISTRY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- We demonstrated the feasibility of using ozonation to enhance the performance of dsDNA binding dye SYBR Green I in the fluorescence measurement of total bacterial load in water. Unlike its membrane permeable but expensive equivalent such as SYTO82 dye, SYBR Green I is many times cheaper but membrane impermeable. Ozonation allowed SYBR Green I dye to permeate the membrane and bind with the dsDNA within by first breaching it. Using E. coli K12 bacteria at serial dilution ratios from 1/1 (980 CFU mL(-1)) to 1/200, we achieved corresponding quantification from 618.7 +/- 9.4 to 68.0 +/- 1.9 RFU (100 to 11.00% normalized RFU). In comparison, plate counting and optical density measurement were only able to quantify up till a serial dilution ratio of 1/50 (40 CFU mL(-1) and 0.0421, respectively). Most importantly with ozonation, the sensitivity of SYBR Green I dye based fluorescence measurement was improved by similar to 140 to 210% as compared to that without ozonation. Given its low electrical power consumption, lab-on-chip compatibility and reagent-less nature, ozonation is highly compatible with portable fluorimeters to realize low-cost monitoring of total bacterial load in water.
- DOI
- 10.1039/d0ra08742d
- Appears in Collections:
- 공과대학 > 환경공학과 > Journal papers
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