Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 손아정 | * |
dc.date.accessioned | 2021-02-25T16:31:48Z | - |
dc.date.available | 2021-02-25T16:31:48Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 2046-2069 | * |
dc.identifier.other | OAK-28888 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/257153 | - |
dc.description.abstract | We demonstrated the feasibility of using ozonation to enhance the performance of dsDNA binding dye SYBR Green I in the fluorescence measurement of total bacterial load in water. Unlike its membrane permeable but expensive equivalent such as SYTO82 dye, SYBR Green I is many times cheaper but membrane impermeable. Ozonation allowed SYBR Green I dye to permeate the membrane and bind with the dsDNA within by first breaching it. Using E. coli K12 bacteria at serial dilution ratios from 1/1 (980 CFU mL(-1)) to 1/200, we achieved corresponding quantification from 618.7 +/- 9.4 to 68.0 +/- 1.9 RFU (100 to 11.00% normalized RFU). In comparison, plate counting and optical density measurement were only able to quantify up till a serial dilution ratio of 1/50 (40 CFU mL(-1) and 0.0421, respectively). Most importantly with ozonation, the sensitivity of SYBR Green I dye based fluorescence measurement was improved by similar to 140 to 210% as compared to that without ozonation. Given its low electrical power consumption, lab-on-chip compatibility and reagent-less nature, ozonation is highly compatible with portable fluorimeters to realize low-cost monitoring of total bacterial load in water. | * |
dc.language | English | * |
dc.publisher | ROYAL SOC CHEMISTRY | * |
dc.title | Ozonation enhancement of low cost double-stranded DNA binding dye based fluorescence measurement of total bacterial load in water | * |
dc.type | Article | * |
dc.relation.issue | 7 | * |
dc.relation.volume | 11 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 3931 | * |
dc.relation.lastpage | 3941 | * |
dc.relation.journaltitle | RSC ADVANCES | * |
dc.identifier.doi | 10.1039/d0ra08742d | * |
dc.identifier.wosid | WOS:000615282100017 | * |
dc.identifier.scopusid | 2-s2.0-85099932925 | * |
dc.author.google | Choi, Jiwon | * |
dc.author.google | Chua, Beelee | * |
dc.author.google | Son, Ahjeong | * |
dc.contributor.scopusid | 손아정(23487252600) | * |
dc.date.modifydate | 20240322131724 | * |