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Polypeptide Thermogel-Filled Silk Tube as a Bioactive Nerve Conduit

Title
Polypeptide Thermogel-Filled Silk Tube as a Bioactive Nerve Conduit
Authors
Patel M.Lee H.J.Kwon O.H.Jeong B.
Ewha Authors
정병문Madhumita Patel
SCOPUS Author ID
정병문scopus
Issue Date
2019
Journal Title
ACS Applied Bio Materials
ISSN
2576-6422JCR Link
Citation
ACS Applied Bio Materials vol. 2, no. 5, pp. 1967 - 1974
Keywords
growth factor deliverymesenchymal stem cellnerve conduitneuronal differentiationsilkthermogel
Publisher
American Chemical Society
Indexed
SCOPUS scopus
Document Type
Article
Abstract
To repair nerve damages, nerve conduits are clinically used. However, when the gap distance is greater than 3 cm, nerve damages remain difficult to repair using current empty nerve conduits. To improve the nerve conduit, a silk nerve conduit (diameter = 5 mm and length = 3 cm) filled with a hydrogel matrix was prepared by injecting a thermogelling aqueous poly(ethylene glycol)-poly(l-alanine) (PEG-PA) solution containing stem cells and neurotropic factors into a silk tube at 37 °C. Successful differentiation of the incorporated stem cells into neuronal cells is a key factor for this system to be used as a nerve conduit. Therefore, neuronal differentiation of the incorporated stem cells in the thermogel-filled conduit system was investigated as a bioactive nerve conduit. The study found that the stem cells exhibited 20 to 60-fold increases in mRNA expression for the neuronal biomarkers of NFM and the glial biomarker of GFAP, in the PEG-PA thermogel-filled silk nerve conduit systems containing neurotropic factors of NGF and BDNF compared to control experiments. This article proved the potential of thermogel as an effective filler of a soft nerve conduit. The PEG-PA thermogel-filled silk conduit system incorporating stem cells and neurotropic factors can be promising as a bioactive nerve conduit system. © 2019 American Chemical Society.
DOI
10.1021/acsabm.9b00026
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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