View : 711 Download: 0
An Activatable AIEgen Probe for High-Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision
- Title
- An Activatable AIEgen Probe for High-Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision
- Authors
- Li, Haidong; Yao, Qichao; Xu, Feng; Li, Yueqing; Kim, Dayeh; Chung, Jeewon; Baek, Gain; Wu, Xiaofeng; Hillman, Prima Fitria; Lee, Eun Young; Ge, Haoying; Fan, Jiangli; Wang, Jingyun; Nam, Sang-Jip; Peng, Xiaojun; Yoon, Juyoung
- Ewha Authors
- 윤주영; 남상집
- SCOPUS Author ID
- 윤주영; 남상집
- Issue Date
- 2020
- Journal Title
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- ISSN
- 1433-7851
1521-3773
- Citation
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION vol. 59, no. 25, pp. 10186 - 10195
- Keywords
- aggregation-induced emission; AIEgens; enzyme activity; nanoprobes; tumor detection
- Publisher
- WILEY-V C H VERLAG GMBH
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Monitoring fluctuations in enzyme overexpression facilitates early tumor detection and excision. An AIEgen probe (DQM-ALP) for the imaging of alkaline phosphatase (ALP) activity was synthesized. The probe consists of a quinoline-malononitrile (QM) core decorated with hydrophilic phosphate groups as ALP-recognition units. The rapid liberation of DQM-OH aggregates in the presence of ALP resulted in aggregation-induced fluorescence. The up-regulation of ALP expression in tumor cells was imaged using DQM-ALP. The probe permeated into 3D cervical and liver tumor spheroids for imaging spatially heterogeneous ALP activity with high spatial resolution on a two-photon microscopy platform, providing the fluorescence-guided recognition of sub-millimeter tumorigenesis. DQM-ALP enabled differentiation between tumor and normal tissue ex vivo and in vivo, suggesting that the probe may serve as a powerful tool to assist surgeons during tumor resection.
- DOI
- 10.1002/anie.202001675
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML