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Systematic Study of Functionalizable, Non-Biofouling Agarose Films with Protein and Cellular Patterns on Glass Slides
- Systematic Study of Functionalizable, Non-Biofouling Agarose Films with Protein and Cellular Patterns on Glass Slides
- Han, Gyeongyeop; Hong, Daehwa; Lee, Bong Soo; Ha, EunRae; Park, Ji Hun; Choi, Insung S.; Kang, Sung Min; Lee, Jungkyu K.
- Ewha Authors
- Issue Date
- Journal Title
- CHEMISTRY-AN ASIAN JOURNAL
- CHEMISTRY-AN ASIAN JOURNAL vol. 12, no. 8, pp. 846 - 852
- bioorganic chemistry; coating techniques; protein and cellular pattern; surface chemistry; thin films
- WILEY-V C H VERLAG GMBH
- SCIE; SCOPUS
- Document Type
- Herein we demonstrate a systematic investigation of chemically functionalizable, non-biofouling agarose films over large-area glass surfaces. Agarose films, prepared with various concentrations of aqueous agarose, were activated by using periodate oxidation to generate aldehyde groups at the termini of the agarose chains. The non-biofouling efficacy and binding capabilities of the activated films were evaluated by using protein and cellular patterning, performed by using a microarrayer, microcontact printing, and micromolding in capillaries. Characterization by using a fluorescence slide scanner and a scanning-probe microscope revealed that the pore sizes of the agarose films played an important role in achieving desirable film performance; the 0.2wt% agarose film exhibited the optimum efficacy in this work.
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