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Development of a rapid method to quantify Salmonella Typhimurium using a combination of MPN with qPCR and a shortened time incubation
- Development of a rapid method to quantify Salmonella Typhimurium using a combination of MPN with qPCR and a shortened time incubation
- Kim, Sun Ae; Park, Si Hong; Lee, Sang In; Ricke, Steven C.
- Ewha Authors
- Issue Date
- Journal Title
- FOOD MICROBIOLOGY
- FOOD MICROBIOLOGY vol. 65, pp. 7 - 18
- Salmonella Typhimurium; Quantification; Detection; Rapid; Simple
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- SCIE; SCOPUS
- Document Type
- A novel method was developed for the specific quantification of S. Typhimurium using a most-probable number (MPN) combined with qPCR and a shortened incubation time (MPN-qPCR-SIT). For S. Typhimurium enumeration, dilutions of samples were transferred into three wells on a microtiter plate and the plate was incubated for 4 h. The S. Typhimurium presence in the wells was identified using a qPCR and populations were determined based on an MPN calculation. The R-2 between the MPN-qPCR-SIT and conventional MPN exhibited a high level of correlation (0.9335-0.9752), suggesting that the MPN-qPCRSIT offers a reliable alternative method for S. Typhimurium quantification. Although plating and qPCR were limited in their ability to detect low levels of S. Typhimurium (e.g. 0.18 log MPN/ml), these levels could be successfully detected with the MPN-qPCR-SIT. Chicken breast samples inoculated with S. Typhimurium were incubated at 0, 4, and 24 h and incubated samples were subjected to microbiome analysis. Levels of Salmonella and Enterobacteriaceae increased significantly with incubation time. The obvious benefits of the MPN-qPCR-SIT are: 1) a further confirmation step is not required, 2) the detection limit is as low as conventional MPN, but 3) is more rapid, requiring approximately 7 h to simultaneously complete quantification. (C) 2017 Elsevier Ltd. All rights reserved.
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