Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김선애 | * |
dc.date.accessioned | 2019-11-19T16:30:25Z | - |
dc.date.available | 2019-11-19T16:30:25Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 0740-0020 | * |
dc.identifier.issn | 1095-9998 | * |
dc.identifier.other | OAK-25956 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251915 | - |
dc.description.abstract | A novel method was developed for the specific quantification of S. Typhimurium using a most-probable number (MPN) combined with qPCR and a shortened incubation time (MPN-qPCR-SIT). For S. Typhimurium enumeration, dilutions of samples were transferred into three wells on a microtiter plate and the plate was incubated for 4 h. The S. Typhimurium presence in the wells was identified using a qPCR and populations were determined based on an MPN calculation. The R-2 between the MPN-qPCR-SIT and conventional MPN exhibited a high level of correlation (0.9335-0.9752), suggesting that the MPN-qPCRSIT offers a reliable alternative method for S. Typhimurium quantification. Although plating and qPCR were limited in their ability to detect low levels of S. Typhimurium (e.g. 0.18 log MPN/ml), these levels could be successfully detected with the MPN-qPCR-SIT. Chicken breast samples inoculated with S. Typhimurium were incubated at 0, 4, and 24 h and incubated samples were subjected to microbiome analysis. Levels of Salmonella and Enterobacteriaceae increased significantly with incubation time. The obvious benefits of the MPN-qPCR-SIT are: 1) a further confirmation step is not required, 2) the detection limit is as low as conventional MPN, but 3) is more rapid, requiring approximately 7 h to simultaneously complete quantification. (C) 2017 Elsevier Ltd. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | * |
dc.subject | Salmonella Typhimurium | * |
dc.subject | Quantification | * |
dc.subject | Detection | * |
dc.subject | Rapid | * |
dc.subject | Simple | * |
dc.title | Development of a rapid method to quantify Salmonella Typhimurium using a combination of MPN with qPCR and a shortened time incubation | * |
dc.type | Article | * |
dc.relation.volume | 65 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 7 | * |
dc.relation.lastpage | 18 | * |
dc.relation.journaltitle | FOOD MICROBIOLOGY | * |
dc.identifier.doi | 10.1016/j.fm.2017.01.013 | * |
dc.identifier.wosid | WOS:000400714500002 | * |
dc.author.google | Kim, Sun Ae | * |
dc.author.google | Park, Si Hong | * |
dc.author.google | Lee, Sang In | * |
dc.author.google | Ricke, Steven C. | * |
dc.contributor.scopusid | 김선애(36143094800) | * |
dc.date.modifydate | 20240322124432 | * |