Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김유리 | * |
dc.date.accessioned | 2019-10-29T16:30:37Z | - |
dc.date.available | 2019-10-29T16:30:37Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 1756-4646 | * |
dc.identifier.other | OAK-25498 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251692 | - |
dc.description.abstract | The prevalence of inflammatory bowel disease (IBD) is increasing worldwide, and the key risk factor is high sugar diet. The objective of this study was to investigate anti-inflammatory effects of turanose on IBD in a dextran sulfate sodium (DSS)-induced colitis mouse model. An amylosucrase from Neisseria sicca (NsiAS) was newly identified and successfully expressed. This enzyme exhibited optimal activity at 45 degrees C and pH 8.5. By utilizing this recombinant enzyme expressed in the E. coli host, turanose yield maximally reached 47% from 2.0 M sucrose. Colitis was induced with two 5-d cycles of 2.5% DSS in drinking water with a 10-d inter-cycle interval. Mice were fed AIN93G diet with 25% and 50% turanose replacement. Improved disease activity index scores, colon length, histopathological features, and myeloperoxidase activity were observed in the turanose supplemented group. Meanwhile, decreases in micro RNA (miR)-21 expression, histone acetylation, expression of pro-inflammatory cytokines, and phosphorylated ERK and STAT3 were observed in colon tissues. Notably, miR-21 and histone acetylation levels were found to positively correlated with expression of tumor necrosis factor a. In conclusion, turanose can attenuate colitis via regulatory effects on miRNA-21 expression and histone acetylation-related proinflammatory mediators. These findings also support further exploration of turanose as a potential therapeutic sugar substitute for patients with IBD. | * |
dc.language | English | * |
dc.publisher | ELSEVIER | * |
dc.subject | Amylosucrase | * |
dc.subject | Neisseria sicca | * |
dc.subject | Turanose | * |
dc.subject | Colitis | * |
dc.subject | Inflammation | * |
dc.subject | microRNA | * |
dc.title | Purification and characterization of turanose, a sucrose isomer and its anti-inflammatory effects in dextran sulfate sodium (DSS)-induced colitis model | * |
dc.type | Article | * |
dc.relation.volume | 63 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | JOURNAL OF FUNCTIONAL FOODS | * |
dc.identifier.doi | 10.1016/j.jff.2019.103570 | * |
dc.identifier.wosid | WOS:000502884600010 | * |
dc.identifier.scopusid | 2-s2.0-85072271315 | * |
dc.author.google | Kim, Eunju | * |
dc.author.google | Bae, Jaehun | * |
dc.author.google | Lee, Jihye | * |
dc.author.google | Shin, Jae-Ho | * |
dc.author.google | Seok, Pu Reum | * |
dc.author.google | Kim, Yuri | * |
dc.author.google | Yoo, Sang-Ho | * |
dc.contributor.scopusid | 김유리(37066214900;55581176400) | * |
dc.date.modifydate | 20240130115050 | * |