NL repository
menu
검색
Library
Browse
Communities & Collections
By Date
Authors
Titles
Subject
My Repository
My Account
Receive email updates
Edit Profile
DSpace at EWHA
자연과학대학
생명과학전공
Journal papers
View : 950 Download: 468
RACK1 interaction with c-Src is essential for osteoclast function
Title
RACK1 interaction with c-Src is essential for osteoclast function
Authors
Park J.H.
;
Jeong E.
;
Lin J.
;
Ko R.
;
Kim J.H.
;
Yi S.
;
Choi Y.
;
Kang I.-C.
;
Lee D.
;
Lee S.Y.
Ewha Authors
이수영
;
이대기
;
박진희
SCOPUS Author ID
이수영
; 이대기
; 박진희
Issue Date
2019
Journal Title
Experimental and Molecular Medicine
ISSN
1226-3613
Citation
Experimental and Molecular Medicine vol. 51, no. 7
Publisher
Nature Publishing Group
Indexed
SCIE; SCOPUS; KCI
Document Type
Article
Abstract
The scaffolding protein receptor for activated C-kinase 1 (RACK1) mediates receptor activator of nuclear factor κΒ ligand (RANKL)-dependent activation of p38 MAPK in osteoclast precursors; however, the role of RACK1 in mature osteoclasts is unclear. The aim of our study was to identify the interaction between RACK1 and c-Src that is critical for osteoclast function. A RACK1 mutant protein (mutations of tyrosine 228 and 246 residues to phenylalanine; RACK1 Y228F/Y246F) did not interact with c-Src. The mutant retained its ability to differentiate into osteoclasts; however, the integrity of the RANKL-mediated cytoskeleton, bone resorption activity, and phosphorylation of c-Src was significantly decreased. Importantly, lysine 152 (K152) within the Src homology 2 (SH2) domain of c-Src is involved in RACK1 binding. The c-Src K152R mutant (mutation of lysine 152 into arginine) impaired the resorption of bone by osteoclasts. These findings not only clarify the role of the RACK1-c-Src axis as a key regulator of osteoclast function but will also help to develop new antiresorption therapies to prevent bone loss-related diseases. © 2019, The Author(s).
DOI
10.1038/s12276-019-0285-4
Appears in Collections:
자연과학대학
>
생명과학전공
>
Journal papers
Files in This Item:
RACK1 interaction with c-Src.pdf
(1.08 MB)
Download
Export
RIS (EndNote)
XLS (Excel)
XML
Show full item record
Find@EWHA
트윗하기
BROWSE
Communities & Collections
By Date
Authors
Titles
Subject