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Sensitive detection of hydroxymethylcytosine levels in normal and neoplastic cells and tissues

Title
Sensitive detection of hydroxymethylcytosine levels in normal and neoplastic cells and tissues
Authors
Krais, Annette M.Park, Yoon JungReifenberger, GuidoMeister, MichaelPlass, ChristophSchmeiser, Heinz H.
Ewha Authors
박윤정
SCOPUS Author ID
박윤정scopus
Issue Date
2019
Journal Title
ELECTROPHORESIS
ISSN
0173-0835JCR Link

1522-2683JCR Link
Citation
ELECTROPHORESIS vol. 40, no. 9, pp. 1293 - 1297
Keywords
Capillary electrophoresis with laser-induced fluorescenceDNA hydroxymethylationGliomaHydroxymethylcytosineLung cancer
Publisher
WILEY
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
A new sensitive analytical method using capillary electrophoresis with laser induced fluorescence (CE-LIF) was applied for the simultaneous detection of DNA methylation and hydroxymethylation levels in human cancers of different origin. DNA hydroxymethylation, measured as 5-hydroxymethylcytosine (5hmC) levels, was decreased in gliomas with mutation in the isocitrate dehydrogenase 1 (IDH1) gene when compared to IDH1-wildtype gliomas. Independent from IDH1 mutation, 5hmC levels were decreased in lung carcinomas when compared to normal lung tissue. Reduced DNA hydroxymethylation was also observed upon dedifferentiation in cultured murine embryonic fibroblasts. Our data show that reduced DNA hydroxymethylation is related to cellular dedifferentiation and can be detected in various types of cancers, independent from the IDH1 mutation status. Quantitative determination of altered 5hmC levels may therefore have potential as a biomarker linked to cellular differentiation and tumorigenesis.
DOI
10.1002/elps.201800523
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신산업융합대학 > 식품영양학과 > Journal papers
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