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Sensitive detection of hydroxymethylcytosine levels in normal and neoplastic cells and tissues
- Title
- Sensitive detection of hydroxymethylcytosine levels in normal and neoplastic cells and tissues
- Authors
- Krais, Annette M.; Park, Yoon Jung; Reifenberger, Guido; Meister, Michael; Plass, Christoph; Schmeiser, Heinz H.
- Ewha Authors
- 박윤정
- SCOPUS Author ID
- 박윤정
- Issue Date
- 2019
- Journal Title
- ELECTROPHORESIS
- ISSN
- 0173-0835
1522-2683
- Citation
- ELECTROPHORESIS vol. 40, no. 9, pp. 1293 - 1297
- Keywords
- Capillary electrophoresis with laser-induced fluorescence; DNA hydroxymethylation; Glioma; Hydroxymethylcytosine; Lung cancer
- Publisher
- WILEY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- A new sensitive analytical method using capillary electrophoresis with laser induced fluorescence (CE-LIF) was applied for the simultaneous detection of DNA methylation and hydroxymethylation levels in human cancers of different origin. DNA hydroxymethylation, measured as 5-hydroxymethylcytosine (5hmC) levels, was decreased in gliomas with mutation in the isocitrate dehydrogenase 1 (IDH1) gene when compared to IDH1-wildtype gliomas. Independent from IDH1 mutation, 5hmC levels were decreased in lung carcinomas when compared to normal lung tissue. Reduced DNA hydroxymethylation was also observed upon dedifferentiation in cultured murine embryonic fibroblasts. Our data show that reduced DNA hydroxymethylation is related to cellular dedifferentiation and can be detected in various types of cancers, independent from the IDH1 mutation status. Quantitative determination of altered 5hmC levels may therefore have potential as a biomarker linked to cellular differentiation and tumorigenesis.
- DOI
- 10.1002/elps.201800523
- Appears in Collections:
- 신산업융합대학 > 식품영양학과 > Journal papers
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