View : 754 Download: 0
Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
- Title
- Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
- Authors
- Gwynne, Lauren; Sedgwick, Adam C.; Gardiner, Jordan E.; Williams, George T.; Kim, Gyoungmi; Lowe, John P.; Maillard, Jean-Yves; Jenkins, A. Toby A.; Bull, Steven D.; Sessler, Jonathan L.; Yoon, Juyoung; James, Tony D.
- Ewha Authors
- 윤주영
- SCOPUS Author ID
- 윤주영
- Issue Date
- 2019
- Journal Title
- FRONTIERS IN CHEMISTRY
- ISSN
- 2296-2646
- Citation
- FRONTIERS IN CHEMISTRY vol. 7
- Keywords
- reaction-based fluorescent probe; alkaline phosphatase; cell imaging; fluorescence; colorimetric
- Publisher
- FRONTIERS MEDIA SA
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
- DOI
- 10.3389/fchem.2019.00255
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML