Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤주영 | * |
dc.date.accessioned | 2019-05-24T16:30:06Z | - |
dc.date.available | 2019-05-24T16:30:06Z | - |
dc.date.issued | 2019 | * |
dc.identifier.issn | 2296-2646 | * |
dc.identifier.other | OAK-24789 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/249841 | - |
dc.description.abstract | A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems. | * |
dc.language | English | * |
dc.publisher | FRONTIERS MEDIA SA | * |
dc.subject | reaction-based fluorescent probe | * |
dc.subject | alkaline phosphatase | * |
dc.subject | cell imaging | * |
dc.subject | fluorescence | * |
dc.subject | colorimetric | * |
dc.title | Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells | * |
dc.type | Article | * |
dc.relation.volume | 7 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | FRONTIERS IN CHEMISTRY | * |
dc.identifier.doi | 10.3389/fchem.2019.00255 | * |
dc.identifier.wosid | WOS:000466564600001 | * |
dc.author.google | Gwynne, Lauren | * |
dc.author.google | Sedgwick, Adam C. | * |
dc.author.google | Gardiner, Jordan E. | * |
dc.author.google | Williams, George T. | * |
dc.author.google | Kim, Gyoungmi | * |
dc.author.google | Lowe, John P. | * |
dc.author.google | Maillard, Jean-Yves | * |
dc.author.google | Jenkins, A. Toby A. | * |
dc.author.google | Bull, Steven D. | * |
dc.author.google | Sessler, Jonathan L. | * |
dc.author.google | Yoon, Juyoung | * |
dc.author.google | James, Tony D. | * |
dc.contributor.scopusid | 윤주영(7403587371) | * |
dc.date.modifydate | 20240118162450 | * |