DSpace at EWHA: Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

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DC Field	Value	Language
dc.contributor.author	윤주영	*
dc.date.accessioned	2019-05-24T16:30:06Z	-
dc.date.available	2019-05-24T16:30:06Z	-
dc.date.issued	2019	*
dc.identifier.issn	2296-2646	*
dc.identifier.other	OAK-24789	*
dc.identifier.uri	https://dspace.ewha.ac.kr/handle/2015.oak/249841	-
dc.description.abstract	A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.	*
dc.language	English	*
dc.publisher	FRONTIERS MEDIA SA	*
dc.subject	reaction-based fluorescent probe	*
dc.subject	alkaline phosphatase	*
dc.subject	cell imaging	*
dc.subject	fluorescence	*
dc.subject	colorimetric	*
dc.title	Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells	*
dc.type	Article	*
dc.relation.volume	7	*
dc.relation.index	SCIE	*
dc.relation.index	SCOPUS	*
dc.relation.journaltitle	FRONTIERS IN CHEMISTRY	*
dc.identifier.doi	10.3389/fchem.2019.00255	*
dc.identifier.wosid	WOS:000466564600001	*
dc.author.google	Gwynne, Lauren	*
dc.author.google	Sedgwick, Adam C.	*
dc.author.google	Gardiner, Jordan E.	*
dc.author.google	Williams, George T.	*
dc.author.google	Kim, Gyoungmi	*
dc.author.google	Lowe, John P.	*
dc.author.google	Maillard, Jean-Yves	*
dc.author.google	Jenkins, A. Toby A.	*
dc.author.google	Bull, Steven D.	*
dc.author.google	Sessler, Jonathan L.	*
dc.author.google	Yoon, Juyoung	*
dc.author.google	James, Tony D.	*
dc.contributor.scopusid	윤주영(7403587371)	*
dc.date.modifydate	20240118162450	*