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High-affinity l-malate transporter DcuE of Actinobacillus succinogenes catalyses reversible exchange of C4-dicarboxylates
- Title
- High-affinity l-malate transporter DcuE of Actinobacillus succinogenes catalyses reversible exchange of C4-dicarboxylates
- Authors
- Rhie M.N.; Cho Y.B.; Lee Y.J.; Kim O.B.
- Ewha Authors
- 김옥빈
- SCOPUS Author ID
- 김옥빈
- Issue Date
- 2019
- Journal Title
- Environmental Microbiology Reports
- ISSN
- 1758-2229
- Citation
- Environmental Microbiology Reports vol. 11, no. 2, pp. 129 - 139
- Publisher
- Wiley-Blackwell
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Actinobacillus succinogenes is a natural succinate producer, which is the result of fumarate respiration. Succinate production from anaerobic growth with C 4 -dicarboxylates requires transporters catalysing uptake and efflux of C 4 -dicarboxylates. Transporter Asuc_1999 (DcuE) found in A. succinogenes belongs to the Dcu family and was considered the main transporter for fumarate respiration. However, deletion of dcuE affected l-malate uptake of A. succinogenes rather than fumarate uptake. DcuE complemented anaerobic growth of Escherichia coli on l-malate or fumarate; thus, the transporter was characterized in E. coli heterologously. Time-dependent uptake and competitive inhibition assays demonstrated that l-malate is the most preferred substrate for uptake by DcuE. The V max of DcuE for l-malate was 20.04 μmol/gDW·min with K m of 57 μM. The V max for l-malate was comparable to that for fumarate, whereas the K m for l-malate was 8 times lower than that for fumarate. The catalytic efficiency of DcuE for l-malate was 7.3-fold higher than that for fumarate, showing high efficiency and high affinity for l-malate. Furthermore, DcuE catalysed the reversible exchange of three C 4 -dicarboxylates – l-malate, fumarate and succinate – but the preferred substrate for uptake was l-malate. Under physiological conditions, the C 4 -dicarboxylates were reduced to succinate. Therefore, DcuE is proposed as the l-malate/succinate antiporter in A. succinogenes. © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd
- DOI
- 10.1111/1758-2229.12719
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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